Construct: ORF TRCN0000465901
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009363.1_s317c1
- Derived from:
- ccsbBroadEn_00098
- DNA Barcode:
- ACACGTCCTAACCAGTCCAGGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARG1 (383)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465901
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 383 | ARG1 | arginase 1 | NM_000045.4 | 100% | 100% | |
| 2 | human | 383 | ARG1 | arginase 1 | NM_001244438.2 | 97.5% | 97.5% | 129_152del |
| 3 | human | 383 | ARG1 | arginase 1 | NM_001369020.1 | 73.6% | 73.6% | 305_306ins255 |
| 4 | human | 383 | ARG1 | arginase 1 | NR_160934.1 | 61.7% | 1_57del;112_113ins73;951_1374del | |
| 5 | mouse | 11846 | Arg1 | arginase, liver | NM_007482.3 | 83.1% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgccaagtcc agaaccatag ggattattgg agctcctttc tcaaagggac 121 agccacgagg aggggtggaa gaaggcccta cagtattgag aaaggctggt ctgcttgaga 181 aacttaaaga acaagagtgt gatgtgaagg attatgggga cctgcccttt gctgacatcc 241 ctaatgacag tccctttcaa attgtgaaga atccaaggtc tgtgggaaaa gcaagcgagc 301 agctggctgg caaggtggca gaagtcaaga agaacggaag aatcagcctg gtgctgggcg 361 gagaccacag tttggcaatt ggaagcatct ctggccatgc cagggtccac cctgatcttg 421 gagtcatctg ggtggatgct cacactgata tcaacactcc actgacaacc acaagtggaa 481 acttgcatgg acaacctgta tctttcctcc tgaaggaact aaaaggaaag attcccgatg 541 tgccaggatt ctcctgggtg actccctgta tatctgccaa ggatattgtg tatattggct 601 tgagagacgt ggaccctggg gaacactaca ttttgaaaac tctaggcatt aaatactttt 661 caatgactga agtggacaga ctaggaattg gcaaggtgat ggaagaaaca ctcagctatc 721 tactaggaag aaagaaaagg ccaattcatc TAAGTTTTGA TGTTGACGGA CTGGACCCAT 781 CTTTCACACC AGCTACTGGC ACACCAGTCG TGGGAGGTCT GACATACAGA GAAGGTCTCT 841 ACATCACAGA AGAAATCTAC AAAACAGGGC TACTCTCAGG ATTAGATATA ATGGAAGTGA 901 ACCCATCCCT GGGGAAGACA CCAGAAGAAG TAACTCGAAC AGTGAACACA GCAGTTGCAA 961 TAACCTTGGC TTGTTTCGGA CTTGCTCGGG AGGGTAATCA CAAGCCTATT GACTACCTTA 1021 ACCCACCTAA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTC 1141 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAC GTCCTAACCA GTCCAGGGGA 1201 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt