Construct: ORF TRCN0000465907
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014257.1_s317c1
- Derived from:
- ccsbBroadEn_10374
- DNA Barcode:
- CTATAGACTTTCTCCCTCCCAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC400710 (400710)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465907
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 400710 | LOC400710 | uncharacterized LOC400710 | NR_138096.1 | 32.9% | 1_114del;481_1112del | |
| 2 | human | 400710 | LOC400710 | uncharacterized LOC400710 | NR_027257.2 | 23.7% | 1_114del;481_1542del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 435
- ORF length:
- 366
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcactagac agtggggccc tcaaggacct ggcaaatggg ggcttggacc 121 tcggatcggc cTCCCACGCG AAGCTTGCTC CCCACCAGCA TCCCCACGTT GGTGGCGACG 181 CTGCCCCGGC CCCACGGATA CTTCCGCGCC TGTCAGACTC CCTGATGAAC TACCCTTCCC 241 AGAGTACCGC GGGAGCTCGG GCTCCTGAGG GCGACGGTCC TCTGATGGCA GATGCGGGAG 301 AAACTCTGGC GTCAGGCGGC CCTCGCGTGG AGCACACGAA GTCGTGGCTT ATTCTGGCTT 361 CAGTATGTGG GGTGGAGAAG GCGATCCACG CAGCTGCGTC TATTTCCTGT GGATCAATCG 421 CAAAATACGT TCTGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 481 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 541 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTATAGACTT TCTCCCTCCC 601 AACAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt