Construct: ORF TRCN0000465909
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002195.1_s317c1
- Derived from:
- ccsbBroadEn_02670
- DNA Barcode:
- CCGATCGTATGCCCGGGCTACAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR182 (11318)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465909
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11318 | GPR182 | G protein-coupled receptor 182 | NM_007264.4 | 100% | 100% | |
| 2 | human | 11318 | GPR182 | G protein-coupled receptor 182 | XM_011537824.3 | 100% | 100% | |
| 3 | human | 11318 | GPR182 | G protein-coupled receptor 182 | XM_011537825.3 | 100% | 100% | |
| 4 | human | 11318 | GPR182 | G protein-coupled receptor 182 | XM_011537827.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1278
- ORF length:
- 1212
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agtgaaaccc agctgggggc ctggcccctc ggagggggtc accgcagtgc 121 ctaccagtga ccttggagag atccacaact ggaccgagct gcttgacctc ttcaaccaca 181 ctttgtctga gtgccacgtg gagctcagcc agagcaccaa gcgcgtggtc ctctttgccc 241 tctacctggc catgtttgtg gttgggctgg tggagaacct cctggtgata tgcgtcaact 301 ggcgcggctc aggccgggca gggctgatga acctctacat cctcaacatg gccatcgcgg 361 acctgggcat tgtcctgtct ctgcccgtgt ggatgctgga ggtcacgctg gactacacct 421 ggctctgggg cagcttctcc tgccgcttca ctcactactt ctactttgtc aacatgtata 481 gcagcatctt cttcctggtg tgcctcagtg tcgaccgcta tgtcaccctc accagcgcct 541 ccccctcctg gcagcgttac cagcaccgag tgcggcgggc catgtgtgca ggcatctggg 601 tcctctcggc catcatcccg ctgcctgagg tggtccacat ccagctggtg gagggccctg 661 agcccatgtg cctcttcatg gcaccttttg aaacgtacag cacctgggcc ctggcggtgg 721 ccctgtccac caccatcctg ggcttcctgc tgcccttccc tctcatcaca gtcttcaatg 781 tgctgacagc ctgccggctg cggcagccag gacaacccaa gagccggcgc cactgcctgc 841 tgctgtgcgc ctacgtggcc gtctttgtca tgtgctggct gccctatcat gtgaccctgc 901 tgctgctcac actgcatggg acccacatct ccctccactg ccacctgGTC CACCTGCTCT 961 ACTTCTTCTA TGATGTCATT GACTGCTTCT CCATGCTGCA CTGTGTCATC AACCCCATCC 1021 TTTACAACTT TCTCAGCCCA CACTTCCGGG GCCGGCTCCT GAATGCTGTA GTCCATTACC 1081 TTCCTAAGGA CCAGACCAAG GCGGGCACAT GCGCCTCCTC TTCCTCCTGT TCCACCCAGC 1141 ATTCCATCAT CATCACCAAG GGTGATAGCC AGCCTGCTGC AGCAGCCCCC CACCCTGAGC 1201 CAAGCCTGAG CTTTCAGGCA CACCATTTGC TTCCAAATAC TTCCCCCATC TCTCCCACTC 1261 AGCCTCTTAC ACCCAGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1321 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1381 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCGATCG TATGCCCGGG 1441 CTACAGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt