Construct: ORF TRCN0000465919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005196.1_s317c1
- Derived from:
- ccsbBroadEn_08791
- DNA Barcode:
- TCGTATGCGATTGTTCAACGGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C12orf10 (60314)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465919
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60314 | C12orf10 | chromosome 12 open reading ... | NM_021640.4 | 99.6% | 99.2% | 11_12delAAinsGC;1046C>T;1058A>G |
| 2 | mouse | 60315 | Myg1 | melanocyte proliferating ge... | NM_021713.2 | 84.2% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg acaccgcttc ctgcgcggcc tcttaacgct gctgctgccg ccgccacccc 121 tgtatacccg gcaccgcatg ctcggtccag agtccgtccc gcccccaaaa cgatcccgca 181 gcaaactcat ggcaccgccc cgaatcggga cgcacaatgg caccttccac tgcgacgagg 241 cactggcatg cgcactgctt cgcctcctgc cggagtaccg ggatgcagag attgtgcgga 301 cccgggatcc cgaaaaactc gcttcctgtg acatcgtggt ggacgtgggg ggcgagtacg 361 accctcggag acaccgatat gaccatcacc agaggtcttt cacagagacc atgagctccc 421 tgtcccctgg gaagccgtgg cagaccaagc tgagcagtgc gggactcatc tatctgcact 481 tcgggcacaa gctgctggcc cagttgctgg gcactagtga agaggacagc atggtgggca 541 ccctctatga caagatgtat gagaactttg tggaggaggt ggatgctgtg gacaatggga 601 tctcccagtg ggcagagggg gagcctcgat atgcactgac cactaccctg agtgcacgag 661 ttgctcgact taatcctacc tggaaccacc ccgaccaaga cactgaggca gggttcaagc 721 gtgcaatgga tctggttcaa gaggagtttc tgcagagatt agatttctac caacacagct 781 ggctgccagc ccgggccttg gtggaagagg cccttgccca gcgattccag gtggacccaa 841 gtggagagat tgtggaactg gcgaaaggtg catgtccctg gaaggagcat CTCTACCACC 901 TGGAATCTGG GCTGTCCCCT CCAGTGGCCA TCTTCTTTGT TATCTACACT GACCAGGCTG 961 GACAGTGGCG AATACAGTGT GTGCCCAAGG AGCCCCACTC ATTCCAAAGC CGGCTGCCCC 1021 TGCCAGAGCC ATGGCGGGGT CTTCGGGACG AGGCCCTGGA CCAGGTCAGT GGGATCCCTG 1081 GCTGCATCTT CGTCCATGCA AGCGGCTTCA TTGGCGGTCA CCGCACCCGA GAGGGTGCCT 1141 TGAGCATGGC CCGTGCCACC TTGGCCCAGC GCTCATACCT CCCACAAATC TCCTACCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAT CGTATGCGAT TGTTCAACGG TCCACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att