Construct: ORF TRCN0000465923
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005021.1_s317c1
- Derived from:
- ccsbBroadEn_02828
- DNA Barcode:
- CGAAAATCCCAACAAGGCAGTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STX12 (23673)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465923
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23673 | STX12 | syntaxin 12 | NM_177424.3 | 100% | 100% | |
| 2 | mouse | 100226 | Stx12 | syntaxin 12 | NM_133887.4 | 89.9% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 894
- ORF length:
- 828
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc atacggtccc ttagacatgt accggaaccc ggggccctcg gggccccagc 121 tccgggactt cagcagcatc atccagacgt gcagcggcaa catccagcgg atcagccaag 181 ccactgctca gataaagaat ttgatgagcc agctaggaac taagcaggac tcaagcaagc 241 tacaggaaaa tctgcaacag ttacaacact ccacaaatca gctcgccaag gaaacaaatg 301 aattgctgaa agaattaggg tccttgcccc ttcccttatc tacttcagaa cagcgccagc 361 agagacttca gaaggaacgc ctcatgaatg acttctctgc agccttaaac aatttccagg 421 ctgtgcagag aagggtatct gaaaaggaaa aggagagtat tgccagagca agagctggat 481 ctcgtctttc tgcagaagag aggcaaagag aggagcagct ggtctcattT GACAGCCATG 541 AGGAGTGGAA CCAGATGCAG AGCCAGGAGG ATGAGGTGGC CATCACTGAG CAGGATTTGG 601 AACTTATTAA AGAAAGAGAA ACGGCAATTC GGCAGCTGGA GGCTGACATT TTGGATGTCA 661 ATCAGATATT TAAAGATTTG GCCATGATGA TCCATGACCA GGGTGATCTG ATTGATAGCA 721 TAGAAGCCAA TGTGGAAAGC TCAGAGGTGC ACGTCGAAAG AGCCACTGAA CAGTTACAGC 781 GAGCTGCTTA CTATCAGAAA AAATCTCGCA AGAAGATGTG TATCCTGGTG CTTGTCCTGT 841 CAGTGATTAT TCTAATCTTG GGACTTATTA TCTGGCTAGT TTATAAAACG AAGTGCCCAA 901 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 961 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1021 TATCTTGTGG AAAGGACGAC GAAAATCCCA ACAAGGCAGT AGTACGCGTT AAGTCgacaa 1081 tcaacctctg gattacaaaa tttgtgaaag att