Construct: ORF TRCN0000465930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009616.1_s317c1
- Derived from:
- ccsbBroadEn_01028
- DNA Barcode:
- TAACAAAGATGTCATATTTCTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOCS2 (4338)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4338 | MOCS2 | molybdenum cofactor synthes... | NM_004531.5 | 100% | 100% | |
| 2 | mouse | 17434 | Mocs2 | molybdenum cofactor synthes... | NM_001113374.1 | 83.4% | 82.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 630
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gagcttggag atcagctcct cgtgcttcag cctggagacg aaattgccgt 121 tatccccccc attagtggag gatagtgctt ttgagccatc taggaaagat atggatgaag 181 ttgaagagaa atctaaagat gttataaact ttactgccga gaaactttca gtagatgaag 241 tctcacagtt ggtgatttcT CCGCTCTGTG GTGCAATATC CCTATTTGTA GGGACTACAA 301 GAAATAACTT TGAAGGGAAA AAAGTCATTA GCTTAGAATA TGAAGCATAT CTACCCATGG 361 CGGAAAATGA AGTCAGAAAG ATTTGTAGTG ACATTAGGCA GAAATGGCCA GTCAAACACA 421 TAGCAGTGTT CCATAGACTT GGCTTGGTTC CAGTGTCAGA AGCAAGCATA ATCATTGCTG 481 TGTCCTCAGC CCACAGAGCT GCATCTCTTG AAGCTGTGAG CTATGCCATT GATACTTTAA 541 AAGCCAAGGT GCCCATATGG AAAAAGGAAA TATACGAAGA GTCATCAACT TGGAAAGGAA 601 ACAAAGAGTG CTTTTGGGCA TCCAACAGTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATAACA 781 AAGATGTCAT ATTTCTGAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt