Construct: ORF TRCN0000465942

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003663.1_s317c1
Derived from:
ccsbBroadEn_04787
DNA Barcode:
TGCTTACAGAATAAAATTAACGGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USH1G (124590)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465942
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 124590 USH1G USH1 protein network compon... NM_173477.5 100% 100%
2 human 124590 USH1G USH1 protein network compon... NM_001282489.3 77.6% 77.6% 0_1ins309
3 human 124590 USH1G USH1 protein network compon... XM_011524296.2 77.6% 77.6% 0_1ins309
4 mouse 16470 Ush1g USH1 protein network compon... NM_176847.3 90.8% 96.3% (many diffs)
5 mouse 16470 Ush1g USH1 protein network compon... XM_017314300.1 70.1% 73.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1452
ORF length:
1383
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaacgaccag taccaccggg cagcccggga tggctacctg gagctcctca
121aggaggccac ccgaaaggag ctgaatgccc ccgacgagga tggcatgacc cccactctct
181gggctgccta ccatggcaac ctcgagtcgc tgcgtctcat tgtgagccgc gggggtgacc
241cggacaagtg tgacatctgg ggcaacacac ccctgcatct ggcagcttcc aatggccact
301tgcactgcct gtccttcctg gtgtccttcg gagccaacat ctggtgccta gacaacgact
361accacacgcc gctggacatg gctgccatga agggccacat ggaatgcgtg cgctacctgg
421actccatcgc ggccaagcag agcagcctca accccaagct ggtgggtaag ctgaaggaca
481aggccttccg cgaggcggag cggcgcatcc gcgagtgcgc caagctgcag cggaggcacc
541acgaacgcat ggagcggcga taccggcgcg agctggccga gcgttccgac accctcagct
601tctccagcct cacgtccagc accctgagcc gccggctgca gcatctggcg ctgggcagcc
661acctgccgta ctctcaggcc acgctgcacg gcacggccag gggcaagacc aagatgcaga
721agaagctgga gcggcgcaag cagggcggcg aaggcacctt caaggtctcc gaggatgggc
781gcaagagcgc ccgctcgctc tcgggcctgc agctgggcag cgacgtgatg ttcgtgcgcc
841agggcaccta cgccaatccc aaggagtggg gccgagcccc gctccgggac atgttcctct
901cggacgagga cagcgtctcc cgtgccacgc tggcggccga gcctgcccac tcggaggtca
961gcaccgactc aggccacgac tccctgttta cccgccccgg cctgggcacc atggtgttcc
1021gcagaaatta cttgagcagt gggctgcacg gactgggccg cgaggatggg ggtctggatg
1081gggtgggagc gccgcggggt cggctgcaga gctcccccag cctggacgat gacagcctgg
1141gcagtgccaa cagcctgcag gaccgcagct gtggggagga gctgccctgg gatgagctcg
1201atttaggctt ggacgaggac ctGGAGCCCG AGACTAGCCC GCTGGAGACC TTCCTGGCCT
1261CTCTGCACAT GGAGGACTTT GCCGCCCTCC TGCGGCAGGA GAAGATCGAC CTCGAGGCTT
1321TGATGCTGTG CTCTGACCTC GACCTCCGCA GCATCAGCGT CCCACTGGGG CCCCGAAAGA
1381AGATCTTGGG GGCCGTGAGG AGGCGGCGGC AGGCGATGGA GCGCCCGCCG GCCCTGGAGG
1441ACACAGAGCT ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1501ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1561CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGC TTACAGAATA AAATTAACGG
1621AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)