Construct: ORF TRCN0000465984
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015133.1_s317c1
- Derived from:
- ccsbBroadEn_10041
- DNA Barcode:
- CACCCTATCGTCGAGGTCGACTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRAMEF5 (343068)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465984
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 343068 | PRAMEF5 | PRAME family member 5 | NM_001013407.2 | 99.4% | 98.7% | (many diffs) |
| 2 | human | 440561 | PRAMEF6 | PRAME family member 6 | NM_001010889.2 | 99.3% | 98.5% | (many diffs) |
| 3 | human | 653619 | PRAMEF15 | PRAME family member 15 | NM_001098376.3 | 98.3% | 97.2% | (many diffs) |
| 4 | human | 645359 | PRAMEF26 | PRAME family member 26 | NM_001306072.1 | 97.8% | 95.6% | (many diffs) |
| 5 | human | 441873 | PRAMEF25 | PRAME family member 25 | NM_001310134.1 | 97.8% | 95.6% | (many diffs) |
| 6 | human | 400735 | PRAMEF4 | PRAME family member 4 | NM_001009611.4 | 97.7% | 95.3% | (many diffs) |
| 7 | human | 343070 | PRAMEF9 | PRAME family member 9 | NM_001010890.2 | 97.6% | 95.8% | (many diffs) |
| 8 | human | 101929983 | PRAMEF27 | PRAME family member 27 | NM_001300891.1 | 97.4% | 95.3% | (many diffs) |
| 9 | human | 440560 | PRAMEF11 | PRAME family member 11 | NM_001146344.3 | 97.2% | 94.9% | (many diffs) |
| 10 | human | 440560 | PRAMEF11 | PRAME family member 11 | XM_011541479.2 | 97.2% | 94.9% | (many diffs) |
| 11 | human | 645425 | PRAMEF20 | PRAME family member 20 | NM_001099852.2 | 91% | 81% | (many diffs) |
| 12 | human | 112268320 | LOC112268320 | PRAME family member 3 | XM_017030184.2 | 82.1% | 68.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1497
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcatccgg actccaccca gactcctgga gcttgcaggg cggagcctgc 121 tgagggacca agccttggcc atgtccaccc tggaggagct gcccacagaa cttttccccc 181 cactgttcct ggaggccttc agcaggagac gctgtgaggc cctgaagctg atggtgcagg 241 cctggccctt ccgccgcctc cctctgaggc ctctgataaa gatgccttgt ctggaggcct 301 tccaagctgt gctcgatggg cttgatgcac tgcttaccca aggggttcat cccaggaggt 361 ggaaacttca agtgctggat ttacaggatg tctgtgagaa cttctggatg gtttggtctg 421 aagctatggc ccatgggtgc ttcctcaatg ccaagaggaa caaaaaacca gtgcaggact 481 gtccaaggat gagaggacgg cagcccttga ctgtgttcgt agaactttgg ctcaagaaca 541 ggactctgga tgaatacctc acctacctcc ttctatgggt caagcagagg aaagatttac 601 tacacctgtg ctgtaagaag ctgaaaattt tgggaatgcc cttccgcaat atcagaagca 661 tcctgaaaat ggtgaaccta gactgtatcc aggaggtgga agtgaattgc aagtgggtac 721 tgcccatcct gacacagttt accccatacc tgggccacat gaggaatctt cagaagctcg 781 ttctctccca catggatgtc tctcgctacg tttccccaga gcagaagaag gagattgtta 841 cccagttcac cactcagttc ctcaagctgt gctgcctcca aaagctttct atgaactctg 901 tttctttcct cgaaggccac ctggaccagc tgctcagctg tctgaagacc tcgttaaagg 961 tcctcacaat aactaactgt gtgcttttgg aatcagactt gaagcatcta tcccagtgcc 1021 cgagtatcag tcaactaaag accctggacc tgagtggcat cagactgacc aattacagtc 1081 ttgtgcctct ccaaattccc ctagaaaaag ttgcagccac ccttgagtac ctggatttag 1141 atgactgtgg catcatagac tcccaagtca acgccatcct gcctgcccTG AGCCGCTGCT 1201 TTGAGCTCAA CACCTTCAGC TTCTGTGGAA ATCCCATCTC CATGGCCACC CTGGAGAACC 1261 TGCTGAGCCA CACAATCATA CTCAAAAACT TATGCGTGGA GCTGTATCCT GCCCTCCGGG 1321 AGAGTTATGA TGCTGATGGT ACTCTCTGCT GGAGCAGATT TGCTCAAATT AGGGCTGAGC 1381 TGATGAAGAG AGTTAGGGAC TTAAGGCACC CCAAGAGGAT CTTGTTCTGT ACTGACTGCT 1441 GCCCTGACTG TGGCAACAGG TCATTTTATG ACCTGGAGGC AGATCAATGC TGCTGTTTGC 1501 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1561 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1621 ATATATCTTG TGGAAAGGAC GACACCCTAT CGTCGAGGTC GACTGCACGC GTTAAGTCga 1681 caatcaacct ctggattaca aaatttgtga aagatt