Construct: ORF TRCN0000465985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011210.1_s317c1
- Derived from:
- ccsbBroadEn_05449
- DNA Barcode:
- GATCTTAATAGATGAAAAAGGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB16-AS1 (339201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 339201 | ASB16-AS1 | ASB16 antisense RNA 1 | NR_049729.1 | 25.4% | 1_979del;1559_2275del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 648
- ORF length:
- 579
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcagggcct cccagcgcac ctcagggcgc actggctgcg ccccgtagtc 121 cagcagtgcg gcgaaaagga cttcaggctc ccagttgggg gagtcctgga cggcctgcag 181 cgcacagtcc atgggcgtgt ggcccgcccc attggggacc tcagcgcggg ccccgtaacg 241 cagcagcagc TCGGCCAGGC CCCCGCAGCC GTTGGCACAA GCGTTGTGCA GCGGCGTGTG 301 GCGCTTGCGC CCGGCCGCCC GGGCATCAGC TCCAGCCTCC AGGAGCCGGC GCGCCGCAGC 361 CTGGTGTCGC CTGCAGCTAC CTGGGCCCTC GGCCCCAGCG CACGCCGTGT TCAGCGCAGT 421 CTCGCCCTGG CTGGTGCGCA GGCCCACGTC GGCGCCATGC TCCAGGTACA GAGCCACATG 481 TTGCTCCAGG CCGCGCGCCG CCGCCACGTG CAGGGGCGTC TCCTGGCTCT CGCCTGCTGC 541 CAGGTTCACC GTCGCTCCTG CTTCCAGCAG CAACTTGGCG CACCTGGGGA CAAGAGAGCA 601 AGGTCCTGAG GAAAATCCTG AAGGCTTGGG ACCCCTTCAG CTTGCTGTTG CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGAGATCTTA ATAGATGAAA AAGGGTTACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt