Construct: ORF TRCN0000465991
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016786.1_s317c1
- Derived from:
- ccsbBroadEn_06942
- DNA Barcode:
- TCAGACGAGTGTAGCCCGCCATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCG5 (6447)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465991
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6447 | SCG5 | secretogranin V | NM_001144757.2 | 99.8% | 99.5% | 452T>C |
| 2 | human | 6447 | SCG5 | secretogranin V | NM_003020.4 | 99.3% | 99% | 373_374insCAG;449T>C |
| 3 | human | 114118903 | ARHGAP11A-SCG5 | ARHGAP11A-SCG5 readthrough | NM_001368319.1 | 33.6% | 33.5% | 1_1242del;1615_1616insCAG;1691T>C |
| 4 | mouse | 20394 | Scg5 | secretogranin V | NM_009162.3 | 87.5% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 702
- ORF length:
- 636
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ctccaggatg gtctctacca tgctatctgg cctactgttt tggctggcat 121 ctggatggac tccagcattt gcttacagcc cccggacccc tgaccgggtc tcagaagcag 181 atatccagag gctgcttcat ggtgttatgg agcaattggg cattgccagg ccccgagtgg 241 aatatccagc tcaccaggcc atgaatcttg tgggccccca gagcattgaa ggtggagctc 301 atgaaggact tcagcatttg ggtccttttg gcaacatccc CAACATCGTG GCAGAGTTGA 361 CTGGAGACAA CATTCCTAAG GACTTTAGTG AGGATCAGGG GTACCCAGAC CCTCCAAATC 421 CCTGTCCTGT TGGAAAAACA GCAGATGATG GATGTCTAGA AAACACCCCT GACACTGCAG 481 AGTTCAGTCG AGAGTTCCAG TTGCACCAGC ATCTCTCTGA TCCGGAACAT GACTATCCAG 541 GCTTGGGCAA GTGGAACAAG AAACTCCTTT ACGAGAAGAT GAAGGGAGGA GAGAGACGAA 601 AGCGGAGGAG TGTCAATCCA TATCTACAAG GACAGAGACT GGATAATGTT GTTGCAAAGA 661 AGTCTGTCCC CCATTTTTCA GATGAGGATA AGGATCCAGA GTACCCAACT TTCTTGTACA 721 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 781 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 841 AGGACGATCA GACGAGTGTA GCCCGCCATT GACGCGTTAA GTCgacaatc aacctctgga 901 ttacaaaatt tgtgaaagat t