Construct: ORF TRCN0000466026
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006647.1_s317c1
- Derived from:
- BRDN0000391270
- DNA Barcode:
- GTGCGACGCCAGGGCGATAACCTA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCAS1 (8537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466026
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001366297.1 | 29.4% | 6.1% | 1_364del;664_1341del |
| 2 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001316361.2 | 28.5% | 5.9% | 1_364del;664_1383del |
| 3 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001323347.1 | 28.5% | 5.9% | 1_364del;664_1383del |
| 4 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260595.3 | 26.9% | 5.6% | 1_364del;664_1476del |
| 5 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_017028111.2 | 26.3% | 6.1% | 1_364del;664_1509del |
| 6 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260594.3 | 26.2% | 6.1% | 1_364del;664_1518del |
| 7 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001366296.1 | 25.7% | 5.9% | 1_364del;664_1551del |
| 8 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001366295.1 | 25.3% | 5.3% | 1_364del;664_1575del |
| 9 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_011529091.3 | 25.2% | 5.3% | 1_364del;664_1584del |
| 10 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_003657.3 | 24.7% | 5.2% | 1_364del;664_1617del |
| 11 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260591.3 | 24.3% | 5.6% | 1_364del;664_1644del |
| 12 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260590.3 | 22.1% | 5.5% | (many diffs) |
| 13 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260589.3 | 21.8% | 4.9% | (many diffs) |
| 14 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001366298.1 | 21.3% | 5.8% | (many diffs) |
| 15 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_011529090.3 | 21.3% | 5.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 504
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcagctcca ataaagctcc agcgaacaaa gacccaagtg agagctggac 121 acttccggtg gcagctggac cggggcagga cacagataaa accccagggc acgccccggc 181 ccaagacaag gtcctctctg ccgccaggga tcccacgctt ctcccaccTG AGACAGGGGG 241 AGCAGGAGGA GAAGCTCCCT CCAAGCCCAA GGACTCCAGC TTTTTTGACA AATTCTTCAA 301 GCTGGACAAG GGACAGGAAA AGGTGCCAGG TGACAGCCAA CAGGAAGCCA AGAGGGCAGA 361 GCATCAAGAC AAGCTCCAAA AGAGACCTGA GAAGCGGCAG CAGTCCCTTG GGGGCTTCTT 421 TAAAGGCCTG GGACCAAAGC GGATGTTGGA TGCTCAAGTG CAAACAGACC CAGTATCCAT 481 CGGACCAGTT GGCAAATCCA AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 541 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 601 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT GCGACGCCAG 661 GGCGATAACC TAACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 721 tt