Transcript: Human XM_005260595.3

PREDICTED: Homo sapiens breast carcinoma amplified sequence 1 (BCAS1), transcript variant X11, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
BCAS1 (8537)
Length:
3115
CDS:
121..1734

Additional Resources:

NCBI RefSeq record:
XM_005260595.3
NBCI Gene record:
BCAS1 (8537)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005260595.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231091 ATAAGTGTCAAGACGGATAAT pLKO_005 274 CDS 100% 13.200 10.560 N BCAS1 n/a
2 TRCN0000218325 AGTCAGACAAAGCCAACTTTA pLKO_005 1220 CDS 100% 13.200 9.240 N BCAS1 n/a
3 TRCN0000231094 GTTGTATATTGAGGTGTATTA pLKO_005 1876 3UTR 100% 13.200 9.240 N BCAS1 n/a
4 TRCN0000130428 GAGGGAATAGAGGAATGTGTT pLKO.1 1999 3UTR 100% 4.950 3.465 N BCAS1 n/a
5 TRCN0000128179 CAAATTCTTCAAGCTGGACAA pLKO.1 705 CDS 100% 4.050 2.835 N BCAS1 n/a
6 TRCN0000130849 GCATCAAGACAAGGTGGATGA pLKO.1 777 CDS 100% 4.050 2.835 N BCAS1 n/a
7 TRCN0000129875 GCTGTTGTATATTGAGGTGTA pLKO.1 1873 3UTR 100% 4.050 2.835 N BCAS1 n/a
8 TRCN0000130206 CAACGGAAAGAATCTTGGGAA pLKO.1 345 CDS 100% 2.640 1.848 N BCAS1 n/a
9 TRCN0000129025 GCTGACCTTAAGTCAGACAAA pLKO.1 1210 CDS 100% 0.495 0.347 N BCAS1 n/a
10 TRCN0000231093 CAGTTGGCAAATCCAAGTAAA pLKO_005 1715 CDS 100% 13.200 7.920 N BCAS1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005260595.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07257 pDONR223 100% 78.1% 78.2% None 858C>T;927_1061del;1275_1276ins276 n/a
2 ccsbBroad304_07257 pLX_304 0% 78.1% 78.2% V5 858C>T;927_1061del;1275_1276ins276 n/a
3 TRCN0000480646 GCATTTGCACTACACCAACGTAGT pLX_317 18.8% 78.1% 78.2% V5 858C>T;927_1061del;1275_1276ins276 n/a
4 ccsbBroadEn_15636 pDONR223 0% 78% 77.9% None (many diffs) n/a
5 ccsbBroad304_15636 pLX_304 0% 78% 77.9% V5 (many diffs) n/a
6 TRCN0000480636 AGGGAGCTTAGAGGAATGATTCAT pLX_317 20.5% 78% 77.9% V5 (many diffs) n/a
7 TRCN0000466026 GTGCGACGCCAGGGCGATAACCTA pLX_317 62.3% 26.9% 5.6% V5 (not translated due to frame shift) 1_364del;664_1476del n/a
Download CSV