Construct: ORF TRCN0000466037
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010620.1_s317c1
- Derived from:
- ccsbBroadEn_10380
- DNA Barcode:
- CTTAATCTAACAGAATCTTCCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM74A1 (401507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466037
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401507 | FAM74A1 | family with sequence simila... | NR_026803.2 | 61.1% | 1_179del;561_623del | |
| 2 | human | 653123 | FAM74A6 | family with sequence simila... | NR_110999.1 | 49% | (many diffs) | |
| 3 | human | 401508 | FAM74A4 | family with sequence simila... | NR_026802.1 | 20.4% | (many diffs) | |
| 4 | human | 728495 | FAM74A3 | family with sequence simila... | NR_026801.1 | 20% | (many diffs) | |
| 5 | human | 100996582 | FAM74A7 | family with sequence simila... | NR_126166.1 | 19.8% | (many diffs) | |
| 6 | human | 401508 | FAM74A4 | family with sequence simila... | NR_110998.1 | 19.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 450
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggagggag ctcagaggct gtcctggagg agacgtggag acagctcaga 121 ggctgtccca gagaagacgt ggaaagagcT CAGAGGCTGT CCCAGAGAAG ACGTGGAGAG 181 CTCAGAGGAT GTCCCAGAGA AGACGTGGAG AGAGTTCAGA GGCTGTCCCG GAGAAGACGT 241 GGAAAGAGCT CAGAAACTCA GAGACTGTCC CGGAGAAGAC ATGGAAACAG CTCAGACGCT 301 GTCTGCAAGA AGATGTGGAG AGAGTTCAGA GGCTGTCGTT ACTGCTGCAC TTGGCTGTTT 361 TTCTTTGGAT AATTATTGCA ATCAATTTTA GTAATTCAGG TGTTAAATCA CAGTCAAGCA 421 CCTATTTACC TAGTGGTAAA ATCCTTAAGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTTAA 601 TCTAACAGAA TCTTCCCAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt