Construct: ORF TRCN0000466085
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010503.1_s317c1
- Derived from:
- ccsbBroadEn_06520
- DNA Barcode:
- GTGATCCAATTTTGGTTGGTCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LEP (3952)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466085
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3952 | LEP | leptin | NM_000230.3 | 99.8% | 99.4% | 56T>C |
| 2 | human | 3952 | LEP | leptin | XM_005250340.5 | 99.2% | 98.8% | 56T>C;143_144insGCA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 567
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ttggggaacc ctgtgcggat tcttgtggct ttggccctat cttttctatg 121 cccaagctgt gcccatccaa aaagtccaag atgacaccaa aaccctcatc aagacaattg 181 tcaccaggat caatgacatt tcacacacgc agtcagtctc ctccaaacag aaagtcaccg 241 gtttggactt cattcctggg ctccacccca tcctgacctt atccaagatg gaccagacac 301 tggcagtcta ccaacagatc ctcaccagta tgccttccag aaacgtgatc caaatatcca 361 acgacctgga gaaccTCCGG GATCTTCTTC ACGTGCTGGC CTTCTCTAAG AGCTGCCACT 421 TGCCCTGGGC CAGTGGCCTG GAGACCTTGG ACAGCCTGGG GGGTGTCCTG GAAGCTTCAG 481 GCTACTCCAC AGAGGTGGTG GCCCTGAGCA GGCTGCAGGG GTCTCTGCAG GACATGCTGT 541 GGCAGCTGGA CCTCAGCCCT GGGTGCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTGATCCA 721 ATTTTGGTTG GTCGTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt