Construct: ORF TRCN0000466091
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010173.1_s317c1
- Derived from:
- ccsbBroadEn_13943
- DNA Barcode:
- CTTTGAAACAGTCTTCAAGCCCTT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHAG (6005)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466091
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6005 | RHAG | Rh associated glycoprotein | NM_000324.3 | 99.7% | 99.2% | 299T>C;593A>G;1224delG |
| 2 | human | 6005 | RHAG | Rh associated glycoprotein | XM_011514788.1 | 51.9% | 51.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1293
- ORF length:
- 1227
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gttcacattc cctctcatgg ctatagtcct ggaaattgcc atgattgttt 121 tatttggatt atttgttgag tatgaaacgg accagactgt tctcgagcag ctcaacatca 181 ccaagccaac agacatgggc atattctttg agttatatcc tctgttccaa gatgtacatg 241 ttatgatatt tgttgggttt ggcttcctca tgaccttcct gaagaaatat ggcttcagca 301 gtgtgggtat caacctactc gttgctgctt tgggcctcca gtggggcact attgtacagg 361 gaaccctgca aagccaggga cagaaattta acattggaat caaaaacatg ataaatgcag 421 acttcagtgc agccacagtt ctgatatctt ttggagctgt cctgggaaaa acgagcccca 481 cccaaatgct gatcatgaca attttagaaa ttgttttctt tgcccacaat gaatacctgg 541 ttagtgaaat atttaaggcc tctgacattg gagcatcaat gacgatccat gcctttgggg 601 cctactttgg cttggctgta gcaggcatct tgtatcgatc tggactgaga aaggggcgtg 661 aaaatgaaga gtccgcatac tactcagact tgtttgcaat gattgggact ctctttctgt 721 ggatgttttg gcccagcttt aactcggcca ttgctgaacc tggagacaaa cagtgcaggg 781 ccattgtaaa cacgtacttc tctctcgctg cctgtgtgct cacagccttt gccttctcca 841 gcctagtgga gcaccgaggc aagctcaaca tggttcacat tcagaatgcc acccttgctg 901 gaggagttgc tgtgggcact tgtgcggata tggcaattca cccatttggt tctatgatta 961 ttgggagcat tgcaggaatg gtctctgtgc ttgGATACAA GTTCCTGACT CCACTTTTTA 1021 CTACTAAACT GAGGATCCAT GATACATGTG GGGTCCATAA CCTCCACGGC TTACCTGGTG 1081 TAGTGGGAGG CCTTGCAGGC ATTGTGGCAG TAGCAATGGG CGCCTCCAAC ACGTCTATGG 1141 CCATGCAGGC AGCTGCACTG GGTTCCTCTA TCGGAACAGC AGTTGTTGGA GGTCTGATGA 1201 CAGGTTTAAT TCTAAAGTTG CCTCTCTGGG GACAGCCATC TGACCAGAAC TGCTATGATG 1261 ATTCTGTTTA TTGGAAGGTC CCTAAGACAG ATACCCAACT TTCTTGTACA AAGTGGTTGA 1321 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1381 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTT 1441 TGAAACAGTC TTCAAGCCCT TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1501 tgtgaaagat t