Construct: ORF TRCN0000466092
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014744.1_s317c1
- Derived from:
- ccsbBroadEn_08309
- DNA Barcode:
- GTCCATGTACCTGACATATATCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHF7 (51533)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466092
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51533 | PHF7 | PHD finger protein 7 | NM_001321126.2 | 99.9% | 100% | 231C>T |
| 2 | human | 51533 | PHF7 | PHD finger protein 7 | NM_001321127.2 | 99.9% | 100% | 231C>T |
| 3 | human | 51533 | PHF7 | PHD finger protein 7 | NM_016483.7 | 99.9% | 100% | 231C>T |
| 4 | human | 51533 | PHF7 | PHD finger protein 7 | XM_024453589.1 | 99.9% | 100% | 231C>T |
| 5 | human | 51533 | PHF7 | PHD finger protein 7 | NM_001278221.3 | 89.6% | 89.7% | 231C>T;679_680ins117 |
| 6 | human | 51533 | PHF7 | PHD finger protein 7 | XM_011533826.3 | 70.4% | 69.8% | (many diffs) |
| 7 | mouse | 71838 | Phf7 | PHD finger protein 7 | NM_027949.1 | 70.2% | 67.9% | (many diffs) |
| 8 | mouse | 71838 | Phf7 | PHD finger protein 7 | XM_006519583.3 | 60.7% | 58.2% | (many diffs) |
| 9 | mouse | 71838 | Phf7 | PHD finger protein 7 | XM_011245202.2 | 52.2% | 49.3% | (many diffs) |
| 10 | mouse | 71838 | Phf7 | PHD finger protein 7 | XM_011245203.2 | 37.4% | 30.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gactgtaaaa gaaaagaagg aatgccagag attgagaaaa tctgccaaga 121 ctaggagggt aacccagagg aaaccgtctt cagggcctgt ttgctggcta tgccttcgag 181 aacctgggga tcccgaaaaa ttaggggaat ttcttcagaa agacaatatc agcgtgcatt 241 atttctgtct tatcttatct agtaagctgc ctcagagggg ccagtccaac agaggtttcc 301 atggatttct gcctgaagac atcaaaaagg aggcagcccg ggcttctagg aagatctgct 361 ttgtgtgcaa gaaaaaggga gctgctatca actgccagaa ggatcagtgc ctcagaaact 421 tccatctgcc ttgtggccaa gaaaggggtt gcctttcaca attttttgga gagtacaaat 481 cattttgtga caaacatcgc ccaacacaga acatccaaca tgggcatgtg ggggaggaaa 541 gctgcatctt atgttgtgaa gacttatccc aacagagtgt tgagaacatc cagagcccgt 601 gttgtagtca agccatctac caccgcaagt gcatacagaa atatgcccac acatcagcaa 661 agcatttctt caaatgtcca cagtgtaaca atcgaaaaga gtttcctcaa gaaatgctga 721 gaatgggaat tcatattcca gacagagatg ctgcctggga actcgagcca ggggctttct 781 cagacttata tcagcgctat cagcactgtg atgcccccat ctgtctgtat gaacaaggca 841 gagacagctt TGAGGATGAA GGGAGGTGGT GCCTCATTCT GTGTGCTACA TGCGGATCCC 901 ACGGAACCCA CAGGGACTGC TCCTCTCTTA GATCTAACAG TAAGAAATGG GAGTGTGAGG 961 AGTGTTCACC TGCTGCAGCC ACAGACTACA TACCTGAAAA CTCAGGGGAC ATCCCTTGCT 1021 GCAGCAGCAC CTTCCACCCT GAGGAACATT TCTGCAGAGA CAACACCTTG GAAGAGAATC 1081 CGGGCCTTTC TTGGACTGAT TGGCCAGAAC CTTCCTTATT AGAAAAGCCA GAGTCCTCTC 1141 GTGGCAGGAG GAGCTACTCC TGGAGGTCCA AGGGTGTCAG AATCACTAAC AGCTGCAAAA 1201 AATCCAAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTCCAT GTACCTGACA TATATCTTAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt