Construct: ORF TRCN0000466098
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002225.1_s317c1
- Derived from:
- ccsbBroadEn_05953
- DNA Barcode:
- AACGCCCTCACGTCCTGCCGCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD3E (916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466098
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 916 | CD3E | CD3e molecule | NM_000733.4 | 99.8% | 100% | 54C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 687
- ORF length:
- 621
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gtcgggcact cactggagag ttctgggcct ctgcctctta tcagttggtg 121 tttgggggca agatggtaat gaagaaatgg gtggtattac acagacacca tataaagtct 181 ccatctctgg aaccacagta atattgacat gccctcagta tcctggatct gaaatactat 241 ggcaacacaa tgataaaaac ataggcggtg atgaggatga taaaaacata ggcagtgatg 301 aggatcacct gtcactgaag gaattttcag aattGGAGCA AAGTGGTTAT TATGTCTGCT 361 ACCCCAGAGG AAGCAAACCA GAAGATGCGA ACTTTTATCT CTACCTGAGG GCAAGAGTGT 421 GTGAGAACTG CATGGAGATG GATGTGATGT CGGTGGCCAC AATTGTCATA GTGGACATCT 481 GCATCACTGG GGGCTTGCTG CTGCTGGTTT ACTACTGGAG CAAGAATAGA AAGGCCAAGG 541 CCAAGCCTGT GACACGAGGA GCGGGTGCTG GCGGCAGGCA AAGGGGACAA AACAAGGAGA 601 GGCCACCACC TGTTCCCAAC CCAGACTATG AGCCCATCCG GAAAGGCCAG CGGGACCTGT 661 ATTCTGGCCT GAATCAGAGA CGCATCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 721 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 781 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACGCCCT 841 CACGTCCTGC CGCATGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 901 aagatt