Construct: ORF TRCN0000466122
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017725.1_s317c1
- Derived from:
- ccsbBroadEn_04488
- DNA Barcode:
- TCCATCAGAACATGCTTGACACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GGACT (87769)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466122
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 87769 | GGACT | gamma-glutamylamine cyclotr... | NM_001195087.1 | 100% | 100% | |
| 2 | human | 87769 | GGACT | gamma-glutamylamine cyclotr... | NM_033110.2 | 100% | 100% | |
| 3 | human | 87769 | GGACT | gamma-glutamylamine cyclotr... | XM_005254083.2 | 100% | 100% | |
| 4 | human | 87769 | GGACT | gamma-glutamylamine cyclotr... | XM_011521129.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctagtcttc gtgtacggca ccctgaagcg gggtcagccc aaccacaggg 121 tcctgcggga cggcgcccac ggctccgcag cctttcgggc gcgcggccgc acgctGGAGC 181 CCTACCCGTT GGTGATCGCG GGGGAGCACA ACATCCCGTG GCTGCTGCAC CTGCCCGGCT 241 CGGGGCGCCT CGTGGAGGGC GAGGTCTACG CGGTAGACGA GCGGATGCTG CGCTTTCTGG 301 ATGACTTCGA GAGTTGCCCG GCCCTGTACC AGCGCACGGT GCTGCGGGTA CAGCTGCTGG 361 AGGACCGGGC CCCGGGCGCA GAGGAGCCGC CAGCGCCCAC CGCGGTGCAG TGCTTCGTGT 421 ACAGCAGGGC CACCTTCCCG CCGGAGTGGG CCCAGCTCCC GCACCATGAC AGCTACGACT 481 CCGAGGGGCC GCACGGGCTG CGCTACAACC CCCGGGAGAA CAGATACCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA TCCATCAGAA CATGCTTGAC ACGAACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt