Construct: ORF TRCN0000466146
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008090.1_s317c1
- Derived from:
- ccsbBroadEn_08362
- DNA Barcode:
- CCGCATGATGTAAAGGCAATGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C21orf91 (54149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466146
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54149 | C21orf91 | chromosome 21 open reading ... | NM_017447.4 | 99.8% | 99.6% | 408C>G |
| 2 | human | 54149 | C21orf91 | chromosome 21 open reading ... | NM_001100420.2 | 99.5% | 99.3% | 408C>G;727_729delGAA |
| 3 | human | 54149 | C21orf91 | chromosome 21 open reading ... | NM_001100421.2 | 74.5% | 74.3% | 408C>G;663_664ins225 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 954
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cgaagaggag cagtttgtaa acattgattt gaatgatgac aacatttgca 121 gtgtttgtaa actgggaaca gacaaagaaa cactctcctt ctgccacatt tgttttgagc 181 taaatattga gggggtacca aagtctgatc tcttgcacac caaatcatta aggggccata 241 aagactgctt tgaaaaatac catttaattg caaaccaggg ttgtcctcga tctaagcttt 301 caaaaagtac ttatgaagaa gttaaaacca ttttgagtaa gaagataaac tggattgtgc 361 agtatgcaca aaataaggat ctggattcag attctgaatg ttctaaaaac ccccagcatc 421 atctgtttaa tttcaggcat aagccagaag aaaaattact cccacagttt gagtcccaag 481 taccaaaata ttctgcaaaa tggatagatg gaagtgcagg tggcatctct aactgtacac 541 aaagaatttt ggagcagagg gaaaatacag actttggact ttctatgtta caagattcag 601 gtgccacttt atgtcgtaac agtgtattgt ggccTCATAG TCACAACCAG GCACAGAAAA 661 AAGAAGAGAC AATCTCTAGT CCAGAGGCTA ATGTCCAGAC CCAGCATCCA CATTACAGCA 721 GAGAGGAATT GAATTCGATG ACTCTTGGTG AGGTAGAGCA ACTGAATGCA AAGCTCCTAC 781 AGCAAATCCA GGTTTTTGAA GAGTTAACTC ACCAAGTGCA AGAAAAAGAT TCTTTGGCCT 841 CACAGCTCCA TGTCCGCCAC GTTGCCATCG AACAGCTTCT GAAGAACTGT TCTAAGTTAC 901 CATGTCTGCA AGTAGGGCGA ACAGGAATGA AGTCGCACCT ACCCATAAAC AACTGCCCAA 961 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1021 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1081 TATCTTGTGG AAAGGACGAC CGCATGATGT AAAGGCAATG CTCACGCGTT AAGTCgacaa 1141 tcaacctctg gattacaaaa tttgtgaaag att