Transcript: Human NM_001100420.2

Homo sapiens chromosome 21 open reading frame 91 (C21orf91), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-04
Taxon:
Homo sapiens (human)
Gene:
C21orf91 (54149)
Length:
5396
CDS:
62..955

Additional Resources:

NCBI RefSeq record:
NM_001100420.2
NBCI Gene record:
C21orf91 (54149)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001100420.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000276519 CTCTTGCACACCAAATCATTA pLKO_005 206 CDS 100% 13.200 10.560 N C21orf91 n/a
2 TRCN0000128081 CTCTAGTCTTCTAGGGCTAAT pLKO.1 1119 3UTR 100% 10.800 8.640 N C21orf91 n/a
3 TRCN0000276520 CTCTAGTCTTCTAGGGCTAAT pLKO_005 1119 3UTR 100% 10.800 8.640 N C21orf91 n/a
4 TRCN0000285585 CAAGATTCAGGTGCCACTTTA pLKO_005 587 CDS 100% 13.200 9.240 N C21orf91 n/a
5 TRCN0000285586 GAAGTCGCACCTACCCATAAA pLKO_005 928 CDS 100% 13.200 9.240 N C21orf91 n/a
6 TRCN0000191634 GCCATAAAGACTGCTTTGAAA pLKO.1 231 CDS 100% 5.625 3.938 N D16Ertd472e n/a
7 TRCN0000128438 GCATCTCTAACTGTACACAAA pLKO.1 519 CDS 100% 4.950 3.465 N C21orf91 n/a
8 TRCN0000127815 GAAGAAAGTTGTGGTCCGTTT pLKO.1 1026 3UTR 100% 4.050 2.835 N C21orf91 n/a
9 TRCN0000312503 GAAGAAAGTTGTGGTCCGTTT pLKO_005 1026 3UTR 100% 4.050 2.835 N C21orf91 n/a
10 TRCN0000128458 GATAAACTGGATTGTGCAGTA pLKO.1 340 CDS 100% 4.050 2.835 N C21orf91 n/a
11 TRCN0000131020 CCACATTACAGCAGAGAGGAA pLKO.1 704 CDS 100% 2.640 1.848 N C21orf91 n/a
12 TRCN0000201296 GCAGTGTTTGTAAACTGGGAA pLKO.1 114 CDS 100% 2.640 1.848 N D16Ertd472e n/a
13 TRCN0000131040 CTCCTACAGCAAATCCAGGTT pLKO.1 770 CDS 100% 2.640 1.848 N C21orf91 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001100420.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08362 pDONR223 100% 99.5% 99.3% None 408C>G;727_729delGAA n/a
2 ccsbBroad304_08362 pLX_304 0% 99.5% 99.3% V5 408C>G;727_729delGAA n/a
3 TRCN0000466146 CCGCATGATGTAAAGGCAATGCTC pLX_317 36.3% 99.5% 99.3% V5 408C>G;727_729delGAA n/a
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