Construct: ORF TRCN0000466153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006465.1_s317c1
- Derived from:
- ccsbBroadEn_11248
- DNA Barcode:
- CTTCTATGCACGCCGCGCGACCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPM2A (7957)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466153
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001368129.2 | 49.7% | 49.7% | 1_267del |
| 2 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001368132.1 | 49.7% | 49.7% | 1_267del |
| 3 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | XM_017011301.1 | 49.7% | 49.7% | 1_267del |
| 4 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | XM_017011302.1 | 49.7% | 49.7% | 1_267del |
| 5 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001360064.2 | 45.5% | 45.5% | 1_315del |
| 6 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001360071.2 | 45.5% | 45.5% | 1_315del |
| 7 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001368131.1 | 45.5% | 45.5% | 1_315del |
| 8 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | XM_011536116.1 | 45.5% | 45.5% | 1_315del |
| 9 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | XM_024446551.1 | 45.5% | 45.5% | 1_315del |
| 10 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_005670.4 | 26.5% | 26.5% | 1_729del |
| 11 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001360057.2 | 24.6% | 1.6% | 1_487del;672_673ins79 |
| 12 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001018041.2 | 21.6% | 20.8% | (many diffs) |
| 13 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NR_153398.2 | 9.8% | 1_302del;567_2680del | |
| 14 | mouse | 13853 | Epm2a | epilepsy, progressive myocl... | NM_010146.2 | 21.7% | 23% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 354
- ORF length:
- 264
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCTGCCCCA GGCGGTGTGC CTGCTGCATG 121 CGCTGCTGGA GAAGGGACAC ATCGTGTACG TGCACTGCAA CGCTGGGGTG GGCCGCTCCA 181 CCGCGGCTGT CTGCGGCTGG CTCCAGTATG TGATGGGCTG GAATCTGAGG AAGGTGCAGT 241 ATTTCCTCAT GGCCAAGAGG CCGGCTGTCT ACATTGACGA AGAGGCCTTG GCCCGGGCAC 301 AAGAAGATTT TTTCCAGAAA TTTGGGAAGG TTCGTTCTTC TGTGTGTAGC CTGTACCCAA 361 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 421 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 481 TATCTTGTGG AAAGGACGAC TTCTATGCAC GCCGCGCGAC CAGACGCGTT AAGTCgacaa 541 tcaacctctg gattacaaaa tttgtgaaag att