Construct: ORF TRCN0000466155
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000923.1_s317c1
- Derived from:
- ccsbBroadEn_10366
- DNA Barcode:
- GTACTTATTGAGCAGGATATCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIR99AHG (388815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466155
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388815 | MIR99AHG | mir-99a-let-7c cluster host... | NR_136543.1 | 7.3% | 1_44del;357_4220del | |
| 2 | human | 388815 | MIR99AHG | mir-99a-let-7c cluster host... | NR_027791.3 | 7.2% | 1_44del;357_4281del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 381
- ORF length:
- 312
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GGAAATTATA TTAGAACTTC AGCAAAGTGC ACAGCTGCAT AATAGAGAAG 121 CCGGCACTCA GATTGACAGG CAAATAAGCC AGCTTGTGGA AGGGGCCTCT GGGTGGCAGC 181 TCTGGGCTTT TGGGTGTTGG ACAACCATAG GCAAAACTGA AATGGAACAG GGGCAGAAAA 241 TAGCACACTT TTACTCTACG CAACTGTTGC TTTTTCATGA TCTTCAGGAA TTTTACTGGG 301 ATAATTATCC AAATAAATTG CAAGCATTCT ATCCAAATGG AGCTCTTTCT GAGATGAAGA 361 GAATTCTCAA TGTCAAGATT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTAC TTATTGAGCA 541 GGATATCGAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt