Construct: ORF TRCN0000466161
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007797.1_s317c1
- Derived from:
- ccsbBroadEn_10818
- DNA Barcode:
- TTATGTGTTGTAGCTGGCACATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FASN (2194)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466161
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2194 | FASN | fatty acid synthase | NM_004104.5 | 17.4% | 17.4% | 1_6216del;6402C>T |
| 2 | human | 2194 | FASN | fatty acid synthase | XM_011523538.2 | 17.4% | 17.4% | 1_6216del;6402C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1383
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caccaacgac acgatcgtca gtggcacgct gccccagcgc atggcgtcct 121 gcctggaggt gctggacctc ttcctgaacc agccccacat ggtcctgagc agctttgtgc 181 tggctgagaa ggctgcggcc tatagggaca gggacagcca gcgggacctg gtggaggccg 241 tggcacacat tctgggcatc cgcgacttgg ctgctgtcaa cctggacagc tcactggcgg 301 acctgggcct ggactcgctc atgagcgtgg aggtgcgcca gacgctggag cgtgagctca 361 acctggtgct gtccgtgcgc gaggtgcggc aactcacgct ccggaaactg caggagctgt 421 cctcaaaggc ggatgaggcc agcgagctgg catgccccac gcccaaggag gatggtctgg 481 cccagcagca gactcagctg aacctgcgct ccctgctggt gaacccggag ggccccaccc 541 tgatgcggct caactccgtg cagagctcgg agcggcccct gttcctggtg cacccaatcg 601 agggctccac caccgtgttc cacagcctgg cctcccggct cagcatcccc acctatggcc 661 tgcagtgcac ccgagctgcg ccccttgaca gcatccacag cctggctgcc tactacatcg 721 actgcatcag gcaggtgcag cccgagggcc cctaccgcgt ggccggctac tcctacgggg 781 cctgcgtggc ctttgaaatg tgctcccagc tgcaggccca gcagagccca gcccccaccc 841 acaacagcct cttcctgttc gacggctcgc ccacctacgt actggcctac acccagagct 901 accgggcaaa gctgacccca ggctgtgagg ctgaggctga gacggaggcc atatgcttct 961 tcgtgcagca gttcacggac atggagcaca acagggtgct GGAGGCGCTG CTGCCGCTGA 1021 AGGGCCTAGA GGAGCGTGTG GCAGCCGCCG TGGACCTGAT CATCAAGAGC CACCAGGGCC 1081 TGGACCGCCA GGAGCTGAGC TTTGCGGCCC GGTCCTTCTA CTACAAGCTG CGTGCCGCTG 1141 AGCAGTACAC ACCCAAGGCC AAGTACCATG GCAACGTGAT GCTACTGCGC GCCAAGACGG 1201 GTGGCGCCTA CGGCGAGGAC CTGGGCGCGG ACTACAACCT CTCCCAGGTA TGCGACGGGA 1261 AAGTATCCGT CCACGTCATC GAGGGTGACC ACCGCACGCT GCTGGAGGGC AGCGGCCTGG 1321 AGTCCATCAT CAGCATCATC CACAGCTCCC TGGCTGAGCC ACGCGTGAGC GTGCGGGAGG 1381 GCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1441 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1501 GGCTTTATAT ATCTTGTGGA AAGGACGATT ATGTGTTGTA GCTGGCACAT ACACGCGTTA 1561 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt