Construct: ORF TRCN0000466172
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002274.1_s317c1
- Derived from:
- ccsbBroadEn_03315
- DNA Barcode:
- AAATTCCCTCGGTGTATTCAGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOP16 (51491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466172
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51491 | NOP16 | NOP16 nucleolar protein | NM_016391.8 | 100% | 100% | |
| 2 | human | 51491 | NOP16 | NOP16 nucleolar protein | NM_001317975.2 | 84.9% | 81.3% | (many diffs) |
| 3 | human | 51491 | NOP16 | NOP16 nucleolar protein | NM_001291307.3 | 77.5% | 77.5% | 391_392ins120 |
| 4 | human | 51491 | NOP16 | NOP16 nucleolar protein | NM_001256539.3 | 56.8% | 55.2% | 391_392ins88;447_696del |
| 5 | human | 51491 | NOP16 | NOP16 nucleolar protein | NM_001256540.4 | 56.5% | 55.2% | 391_392ins91;444_693del |
| 6 | human | 51491 | NOP16 | NOP16 nucleolar protein | NM_001291308.3 | 55.6% | 43.8% | 215_216ins70;297_298ins167 |
| 7 | human | 51491 | NOP16 | NOP16 nucleolar protein | XM_011534567.1 | 55.6% | 43.8% | 215_216ins70;297_298ins167 |
| 8 | human | 51491 | NOP16 | NOP16 nucleolar protein | NR_134316.2 | 54.1% | (many diffs) | |
| 9 | human | 51491 | NOP16 | NOP16 nucleolar protein | NR_134318.2 | 45.6% | (many diffs) | |
| 10 | human | 51491 | NOP16 | NOP16 nucleolar protein | NM_001291305.3 | 45.2% | 29.3% | (many diffs) |
| 11 | mouse | 28126 | Nop16 | NOP16 nucleolar protein | NM_178605.4 | 88.2% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caaggccaag ggcaaaaccc ggaggcagaa gtttggttac agtgtcaacc 121 gaaagcgtct gaaccggaat gctcgacgga aggcagcgcc gcggatcgaa tgctcccaca 181 tccgacatgc ctgggaccac gctaaatcgg tacggcagaa cctggccgag atggggttgg 241 ctgtggaccc caacagggcg gtgcccctcc gtaagagaaa ggtgaaggcc atggaggtgG 301 ACATAGAGGA GAGGCCTAAA GAGCTTGTAC GGAAGCCCTA TGTGCTGAAT GACCTGGAGG 361 CAGAAGCCAG CCTTCCAGAA AAGAAAGGAA ATACTCTGTC TCGGGACCTC ATTGACTATG 421 TACGCTACAT GGTAGAGAAC CACGGGGAGG ACTATAAGGC CATGGCCCGT GATGAGAAGA 481 ATTACTATCA AGATACCCCA AAACAGATTC GGAGTAAGAT CAACGTCTAT AAACGCTTTT 541 ACCCAGCAGA GTGGCAAGAC TTCCTCGATT CTTTGCAGAA GAGGAAGATG GAGGTGGAGT 601 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGAAAATT CCCTCGGTGT ATTCAGTAGA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt