Construct: ORF TRCN0000466192

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012620.2_s317c1
Derived from:
ccsbBroadEn_14924
DNA Barcode:
TCGATCCAGTTAACCTTACAAGCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PAPSS2 (9060)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466192
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9060 PAPSS2 3'-phosphoadenosine 5'-phos... NM_004670.4 100% 100%
2 human 9060 PAPSS2 3'-phosphoadenosine 5'-phos... NM_001015880.2 99.1% 99.1% 864_878del
3 mouse 23972 Papss2 3'-phosphoadenosine 5'-phos... NM_001201470.1 86.6% 93.1% (many diffs)
4 mouse 23972 Papss2 3'-phosphoadenosine 5'-phos... XM_006527065.2 86.1% 91.8% (many diffs)
5 mouse 23972 Papss2 3'-phosphoadenosine 5'-phos... XM_006527064.3 85.9% 92.4% (many diffs)
6 mouse 23972 Papss2 3'-phosphoadenosine 5'-phos... NM_011864.3 85.5% 91.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1908
ORF length:
1842
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ggggatcaag aagcaaaaga cggagaacca gcagaaatcc accaatgtag
121tctatcaggc ccaccatgtg agcaggaata agagagggca agtggttgga acaaggggtg
181ggttccgagg atgtaccgtg tggctaacag gtctctctgg tgctggaaaa acaacgataa
241gttttgccct ggaggagtac cttgtctccc atgccatccc ttgttactcc ctggatgggg
301acaatgtccg tcatggcctt aacagaaatc tcggattctc tcctggggac agagaggaaa
361atatccgccg gattgctgag gtggctaagc tgtttgctga tgctggtctg gtctgcatta
421ccagctttat ttctccattc gcaaaggatc gtgagaatgc ccgcaaaata catgaatcag
481cagggctgcc attctttgaa atatttgtag atgcacctct aaatatttgt gaaagcagag
541acgtaaaagg cctctataaa agggccagag ctggggagat taaaggattt acaggtattg
601attctgatta tgagaaacct gaaactcctg agcgtgtgct taaaaccaat ttgtccacag
661tgagtgactg tgtccaccag gtagtggaac ttctgcaaga gcagaacatt gtaccctata
721ctataatcaa agatatccac gaactctttg tgccggaaaa caaacttgac cacgtccgag
781ctgaggctga aactctccct tcattatcaa ttactaagct ggatctccag tgggtccagg
841ttttgagcga aggctgggcc actcccctca aaggtttcat gcgggagaag gagtacttac
901aggttatgca ctttgacacc ctgctagatg atggcgtgat caacatgagc atccccattg
961tactgcccgt ctctgcagag gataagacac ggctggaagg gtgcagcaag tttgtcctgg
1021cacatggtgg acggagggta gctatcttac gagacgctga attctatgaa cacagaaaag
1081aggaacgctg ttcccgtgtt tgggggacaa catgtacaaa acacccccat atcaaaatgg
1141tgatggaaag tggggactgg ctggttggtg gagaccttca ggtgctggag aaaataagat
1201ggaatgatgg gctggaccaa taccgtctga cacctctgga gctcaaacag aaatgtaaag
1261aaatgaatgc tgatgcggtg tttgcattcc agttgcgcaa tcctgtccac aatggccatg
1321ccctgttgat gcaggacact cgccgcaggc tcctagagag gggctacaag cacccggtcc
1381tcctactaca ccctctgggc ggctggacca aggatgacga tgtgcctcta gactggcgga
1441tgaagcagca cgcggctgtg ctcgaggaag gggtcctgga tcccaagtca accattgttg
1501ccatctttcc gtctcccatg ttatatgctg gccccacaga ggtccagtgg cactgcaggt
1561cccggatgat tgcgggtgcc aatttctaca ttgtgggGAG GGACCCTGCA GGAATGCCCC
1621ATCCTGAAAC CAAGAAGGAT CTGTATGAAC CCACTCATGG GGGCAAGGTC TTGAGCATGG
1681CCCCTGGCCT CACCTCTGTG GAAATCATTC CATTCCGAGT GGCTGCCTAC AACAAAGCCA
1741AAAAAGCCAT GGACTTCTAT GATCCAGCAA GGCACAATGA GTTTGACTTC ATCTCAGGAA
1801CTCGAATGAG GAAGCTCGCC CGGGAAGGAG AGAATCCCCC AGATGGCTTC ATGGCCCCCA
1861AAGCATGGAA GGTCCTGACA GATTATTACA GGTCCCTGGA GAAGAACTAC CCAACTTTCT
1921TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1981CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2041GTGGAAAGGA CGATCGATCC AGTTAACCTT ACAAGCGACG CGTTAAGTCg acaatcaacc
2101tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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