Construct: ORF TRCN0000466204
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010649.1_s317c1
- Derived from:
- ccsbBroadEn_05745
- DNA Barcode:
- CTTAAACAGTACTTCGGATAGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD24 (100133941)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466204
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100133941 | CD24 | CD24 molecule | NM_001291737.1 | 100% | 100% | |
| 2 | human | 100133941 | CD24 | CD24 molecule | NM_001291738.1 | 100% | 100% | |
| 3 | human | 100133941 | CD24 | CD24 molecule | NM_001359084.1 | 100% | 100% | |
| 4 | human | 100133941 | CD24 | CD24 molecule | NM_013230.3 | 100% | 100% | |
| 5 | human | 100133941 | CD24 | CD24 molecule | NM_001291739.1 | 55.2% | 44.5% | (many diffs) |
| 6 | human | 100133941 | CD24 | CD24 molecule | XM_024446293.1 | 14.6% | 12.2% | 1_307del;387_388ins160 |
| 7 | human | 100133941 | CD24 | CD24 molecule | NR_117089.1 | 9.4% | (many diffs) | |
| 8 | human | 100133941 | CD24 | CD24 molecule | NR_117090.1 | 9.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 306
- ORF length:
- 240
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGG CAGAGCAATG GTGGCCAGGC TCGGGCTGGG GCTGCTGCTG CTGGCACTGC 121 TCCTACCCAC GCAGATTTAT TCCAGTGAAA CAACAACTGG AACTTCAAGT AACTCCTCCC 181 AGAGTACTTC CAACTCTGGG TTGGCCCCAA ATCCAACTAA TGCCACCACC AAGGCGGCTG 241 GTGGTGCCCT GCAGTCAACA GCCAGTCTCT TCGTGGTCTC ACTCTCTCTT CTGCATCTCT 301 ACTCTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 361 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 421 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTTAAACAG TACTTCGGAT AGCAAACGCG 481 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt