Construct: ORF TRCN0000466205
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006560.1_s317c1
- Derived from:
- ccsbBroadEn_03777
- DNA Barcode:
- TTTTTTTAATATGCCATAACCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLSCR4 (57088)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466205
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | NM_001128304.2 | 100% | 100% | |
| 2 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | NM_001128305.2 | 100% | 100% | |
| 3 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | NM_020353.3 | 100% | 100% | |
| 4 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | XM_005247654.2 | 100% | 100% | |
| 5 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | XM_005247655.2 | 100% | 100% | |
| 6 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | XM_011513031.2 | 100% | 100% | |
| 7 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | XM_024453686.1 | 100% | 100% | |
| 8 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | NM_001128306.1 | 72.6% | 72.6% | 352_353ins270 |
| 9 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | XM_017006921.1 | 72.6% | 72.6% | 352_353ins270 |
| 10 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | XM_024453687.1 | 72.6% | 72.6% | 352_353ins270 |
| 11 | human | 57088 | PLSCR4 | phospholipid scramblase 4 | NM_001177304.2 | 68% | 68% | 0_1ins45;307_308ins270 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1053
- ORF length:
- 987
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aggtgtggta cccacagccc ctgaacagcc tgcaggtgaa atggaaaatc 121 aaacaaaacc accagatcca aggcctgatg ctcctcctga atacaattct cattttttac 181 caggaccccc tggaacagct gtccctccac ctactggcta cccaggaggc ttgcctatgg 241 gatactacag tccacagcaa cccagtacct tccctttgta ccagccagtt ggtggtatcc 301 atcctgtccg gtatcagcct ggcaaatatc ctatgccaaa tcagtctgtt ccaataacat 361 ggatgccagg gccaactcct atggcaaact gccctcctgg tctggaatac ttagttcagt 421 tggacaacat acatgttctt cagcattttg agcctctgga aatgatgaca tgttttgaaa 481 ctaataatag atatgatatt aaaaacaact cagaccagat ggtttacatt gtaaccgaag 541 acacagatga ctttaccagg aatgcctatc ggacactaag gcccttcgtc ctccgggtca 601 ctgattgtat gggccgagaa atcatgacaa tgcagagacc cttcagatgc acctgctgtt 661 gcttctgttg cccctctgcc agacaagagc tggaggtgca gtgtcctcct ggtgtcacca 721 ttggcttTGT TGCGGAACAT TGGAACCTGT GCAGGGCGGT GTACAGCATC CAAAATGAGA 781 AGAAAGAAAA TGTGATGAGA GTTCGTGGGC CATGCTCAAC CTATGGCTGT GGTTCAGATT 841 CTGTTTTTGA GGTCAAATCC CTTGATGGCA TATCCAACAT CGGCAGTATT ATCCGGAAGT 901 GGAATGGTTT GTTATCAGCA ATGGCAGATG CTGACCATTT TGACATTCAC TTCCCACTAG 961 ACCTGGATGT GAAGATGAAA GCCATGATTT TTGGAGCTTG CTTCCTCATT GACTTCATGT 1021 ATTTTGAAAG ATCTCCACCA CAACGTTCAA GATACCCAAC TTTCTTGTAC AAAGTGGTTG 1081 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1141 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1201 TTTTTAATAT GCCATAACCC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1261 ttgtgaaaga tt