Construct: ORF TRCN0000466213
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008876.1_s317c1
- Derived from:
- ccsbBroadEn_04911
- DNA Barcode:
- TTGAAGAAATGTACTAGGCACCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM71 (137835)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466213
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 137835 | TMEM71 | transmembrane protein 71 | NM_144649.3 | 100% | 100% | |
| 2 | human | 137835 | TMEM71 | transmembrane protein 71 | NM_001364885.2 | 93.2% | 93.2% | 313_369del;486_487insGCA |
| 3 | human | 137835 | TMEM71 | transmembrane protein 71 | NM_001145153.2 | 72.2% | 72.2% | 313_369del;486_487ins189 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 894
- ORF length:
- 828
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta ccgaatatct caactgatgt caacaccagt agcaagttct tccaggttgg 121 aaagagaata tgctggagag ctgtctccca cgtgcatttt cccaagtttc acctgtgatt 181 ccctggatgg ttaccattct tttgaatgcg gctccataga tcccctgaca ggctcccact 241 atacctgtcg ccgaagtccc agactcctca ccaatggcta ctatatttgg actgaagaca 301 gcttcctgtg cgacaaagat ggcaacataa ctctgaaccc atcccagacc agcgttatgt 361 ataaggagaa cttagttagt acctccaaat cttggctgca tggaagtatc tttggtgaca 421 tcaactcttc tccaagtgaa gacaactggt tgaaggggac caggaggttg gacacagacc 481 attgcaatgg aaatgcagat gatttagact gttcttctct gactgatgac tgggagtcag 541 ggaagatgaa tgcagagtct gtgatcacct cctcttccag ccacatcata tctcagccTC 601 CTGGAGGAAA CTCCCATAGC TTGTCTCTTC AGTCCCAGTT GACAGCTTCT GAACGTTTCC 661 AAGAGAATAG TTCGGATCAT TCAGAAACCA GGTTGTTGCA AGAGGTCTTC TTTCAGGCAA 721 TCCTGCTTGC TGTGTGCTTA ATCATTTCTG CATGTGCAAG ATGGTTTATG GGAGAAATAT 781 TAGCCAGTGT CTTCACATGC TCATTGATGA TAACTGTAGC TTATGTGAAA TCATTGTTTC 841 TCAGCCTTGC CAGCTATTTC AAAACCACTG CCTGTGCTCG GTTTGTCAAA ATTTGCCCAA 901 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 961 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1021 TATCTTGTGG AAAGGACGAT TGAAGAAATG TACTAGGCAC CATACGCGTT AAGTCgacaa 1081 tcaacctctg gattacaaaa tttgtgaaag att