Construct: ORF TRCN0000466218
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000663.1_s317c1
- Derived from:
- ccsbBroadEn_12453
- DNA Barcode:
- TTCTTAGGTGGAGGGTGAGCGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NECAB1 (64168)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466218
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64168 | NECAB1 | N-terminal EF-hand calcium ... | NM_022351.5 | 28.3% | 28.2% | 1_753del;783A>G |
| 2 | human | 64168 | NECAB1 | N-terminal EF-hand calcium ... | XM_011517213.2 | 21.5% | 18.5% | (many diffs) |
| 3 | mouse | 69352 | Necab1 | N-terminal EF-hand calcium ... | NM_178617.4 | 25.6% | 27.5% | (many diffs) |
| 4 | mouse | 69352 | Necab1 | N-terminal EF-hand calcium ... | XM_006538234.1 | 24% | 25.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 390
- ORF length:
- 300
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCTTGTGCA GCGGCAGATG TCTGTGATGG 121 AAGAGGACCT GGAAGAATTC CAGCTCGCTC TGAAACACTA CGTGGAGAGT GCTTCCTCCC 181 AAAGTGGATG CTTGCGTATT TCTATACAGA AGCTTTCAAA TGAATCTCGC TACATGATCT 241 ATGAGTTCTG GGAGAATAGT AGTGTATGGA ATAGCCACCT TCAGACAAAT TATAGCAAGA 301 CATTCCAAAG AAGTAATGTG GATTTCTTGG AAACTCCAGA ACTCACATCT ACAATGCTAG 361 TTCCTGCTTC GTGGTGGATC CTGAACAACT ACCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTCTT 541 AGGTGGAGGG TGAGCGTACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt