Construct: ORF TRCN0000466221
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014234.1_s317c1
- Derived from:
- ccsbBroadEn_05015
- DNA Barcode:
- GCACCCTCTATACCCCATTCGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TLCD4 (148534)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466221
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148534 | TLCD4 | TLC domain containing 4 | NM_001199679.1 | 100% | 100% | |
| 2 | human | 148534 | TLCD4 | TLC domain containing 4 | NM_152487.3 | 100% | 100% | |
| 3 | human | 100527978 | TLCD4-RWDD3 | TLCD4-RWDD3 readthrough | NM_001199691.1 | 70.3% | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 858
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagatcaac acaaaactgc tcatcagtgt tacctgtatc agctttttca 121 cctttcagct tcttttctac tttgtaagtt actggttttc agcaaaagtt tctccaggtt 181 tcaatagtct cagcttcaaa aagaagattg aatggaactc aagggtagta tccacatgcc 241 attctttggt ggttggtatt tttggcctgt acattttctt attcgatgag gctactaaag 301 ctgatccact ttggggtggt ccatcacttg caaacgtgaa tattgctatt gcctcaggct 361 acctcatttc tgatttgtcc attataattt tgtattggaa agtgattggt gacaaatttt 421 ttataatgca tcattgtgcg tccctgtatg catactacct tgtactgaaa aatggagtgc 481 tggcatacat tgggaattTT CGCCTGCTTG CAGAGCTTTC CAGCCCGTTT GTGAATCAGC 541 GGTGGTTCTT TGAAGCTCTG AAGTATCCCA AGTTTTCTAA AGCTATCGTT ATCAATGGAA 601 TACTCATGAC AGTAGTATTC TTCATCGTGC GGATTGCCTC AATGCTTCCT CATTATGGCT 661 TCATGTATTC CGTGTATGGA ACAGAACCCT ACATAAGGCT TGGAGTTTTA ATCCAGTTAT 721 CCTGGGTCAT TAGTTGTGTT GTTTTGGATG TGATGAATGT CATGTGGATG ATCAAAATTT 781 CAAAAGGTTG CATCAAAGTC ATCTCTCACA TCAGACAAGA GAAAGCCAAA AATAGTCTTC 841 AGAATGGAAA ACTTGATTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCACCCT CTATACCCCA 1021 TTCGTTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt