Construct: ORF TRCN0000466236

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017270.1_s317c1
Derived from:
ccsbBroadEn_08671
DNA Barcode:
ACTTAGTCACAGATACCTCCGCAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HMCES (56941)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466236
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56941 HMCES 5-hydroxymethylcytosine bin... NM_001006109.1 99.9% 99.7% 180G>T
2 human 56941 HMCES 5-hydroxymethylcytosine bin... NM_001370343.1 99.9% 99.7% 180G>T
3 human 56941 HMCES 5-hydroxymethylcytosine bin... NM_001370344.1 99.9% 99.7% 180G>T
4 human 56941 HMCES 5-hydroxymethylcytosine bin... NM_020187.3 99.9% 99.7% 180G>T
5 human 56941 HMCES 5-hydroxymethylcytosine bin... NM_001363881.1 88% 87.8% 180G>T;324_325ins126
6 human 56941 HMCES 5-hydroxymethylcytosine bin... NM_001370345.1 86.3% 86.1% 180_180delGins145
7 mouse 232210 Hmces 5-hydroxymethylcytosine (hm... NM_173737.2 84.5% 83.6% (many diffs)
8 mouse 232210 Hmces 5-hydroxymethylcytosine (hm... XR_377441.3 58.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1128
ORF length:
1062
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg tgggcgaaca tcctgtcact tacctagaga tgttctcacg agagcttgcg
121cctaccagga tcggcggggc cagcagcggc tcccggagtg gagggaccct gataagtact
181gcccctctta caacaagagt cctcaatcca acagcccagt gcttctgtct cgactgcact
241ttgataagga tgcagactca tctgagcgta tcattgctcc catgcgctgg ggcttggtcc
301cttcttggtt caaagaaagt gatccttcca agctgcagtt caatactacc aactgtcgta
361gtgataccgt aatggagaaa cggtcattta aggtgcctct gggaaaggga agacgctgtg
421tcgttttagc agatggattc tatgagtggc agcgatgtca gggaacaaac cagaggcagc
481catacttcat ctattttcct caaatcaaga cagagaagtc aggtagcatt ggtgctgcag
541atagtcctga gaactgggag aaagtctggg acaactggag gctgctgaca atggccggga
601tctttgactg ctgggagccc ccagagggag gagatgtcct gtattcctat accatcatca
661cagtggattc ctgcaaaggc ttgagtgaca tccaccacag gatgcctgcc atattagatg
721gagaggaggc agtttctaaa tggcttgact ttggtgaagt ctcaactcag gaagctctga
781aattaatcca cccaacagag aacatcaccT TCCATGCAGT CTCTTCTGTG GTGAACAACT
841CGCGAAACAA CACTCCTGAG TGTCTGGCTC CTGTCGACTT GGTGGTCAAA AAGGAGCTCA
901GGGCAAGTGG CAGTAGCCAG AGGATGTTGC AGTGGTTGGC CACAAAGTCA CCCAAAAAGG
961AAGACTCAAA AACACCTCAA AAGGAAGAGT CAGATGTTCC CCAGTGGTCC AGTCAGTTCC
1021TGCAGAAGAG TCCACTCCCC ACCAAGAGAG GCACTGCAGG ACTCCTAGAG CAATGGCTGA
1081AGCGGGAGAA GGAGGAGGAA CCTGTGGCCA AGCGTCCTTA CAGCCAGTGC CCAACTTTCT
1141TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1201CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1261GTGGAAAGGA CGAACTTAGT CACAGATACC TCCGCACACG CGTTAAGTCg acaatcaacc
1321tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)