Transcript: Human NM_001363881.1

Homo sapiens 5-hydroxymethylcytosine binding, ES cell specific (HMCES), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-09
Taxon:
Homo sapiens (human)
Gene:
HMCES (56941)
Length:
1495
CDS:
89..1027

Additional Resources:

NCBI RefSeq record:
NM_001363881.1
NBCI Gene record:
HMCES (56941)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001363881.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000130232 CGTAATGGAGAAACGGTCATT pLKO.1 391 CDS 100% 4.950 6.930 N HMCES n/a
2 TRCN0000330409 CGTAATGGAGAAACGGTCATT pLKO_005 391 CDS 100% 4.950 6.930 N HMCES n/a
3 TRCN0000147137 CTCTGAAATTAATCCACCCAA pLKO.1 672 CDS 100% 2.640 3.696 N HMCES n/a
4 TRCN0000330411 ACAGGATGCCTGCCATATTAG pLKO_005 594 CDS 100% 13.200 10.560 N HMCES n/a
5 TRCN0000330341 ACTCATCTGAGCGTATCATTG pLKO_005 279 CDS 100% 10.800 7.560 N HMCES n/a
6 TRCN0000330340 GATAATAGGTTCTTAACATTG pLKO_005 1076 3UTR 100% 10.800 7.560 N HMCES n/a
7 TRCN0000149845 CTACCAACTGTCGTAGTGATA pLKO.1 369 CDS 100% 4.950 3.465 N HMCES n/a
8 TRCN0000128119 CCTAGAGATGTTCTCACGAGA pLKO.1 116 CDS 100% 2.640 1.848 N HMCES n/a
9 TRCN0000130708 GAGATGTTCTCACGAGAGCTT pLKO.1 120 CDS 100% 2.640 1.848 N HMCES n/a
10 TRCN0000330338 GAGATGTTCTCACGAGAGCTT pLKO_005 120 CDS 100% 2.640 1.848 N HMCES n/a
11 TRCN0000149454 GAGGCAGTTTCTAAATGGCTT pLKO.1 623 CDS 100% 2.640 1.848 N HMCES n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001363881.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15931 pDONR223 0% 88.1% 88.1% None 324_325ins126 n/a
2 ccsbBroad304_15931 pLX_304 0% 88.1% 88.1% V5 324_325ins126 n/a
3 TRCN0000471256 GCTACTACTCTTGACTGACGAACT pLX_317 30.4% 88.1% 88.1% V5 324_325ins126 n/a
4 ccsbBroadEn_08671 pDONR223 100% 88% 87.8% None 180G>T;324_325ins126 n/a
5 ccsbBroad304_08671 pLX_304 0% 88% 87.8% V5 180G>T;324_325ins126 n/a
6 TRCN0000466236 ACTTAGTCACAGATACCTCCGCAC pLX_317 30.4% 88% 87.8% V5 180G>T;324_325ins126 n/a
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