Construct: ORF TRCN0000466248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013799.1_s317c1
- Derived from:
- ccsbBroadEn_00684
- DNA Barcode:
- ATTTCCTGCGCAACTCTCGGAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPS2 (2874)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466248
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2874 | GPS2 | G protein pathway suppressor 2 | NM_004489.5 | 100% | 100% | |
| 2 | mouse | 56310 | Gps2 | G protein pathway suppressor 2 | NM_019726.3 | 91.1% | 97.5% | (many diffs) |
| 3 | mouse | 56310 | Gps2 | G protein pathway suppressor 2 | XM_006533787.2 | 91.1% | 97.5% | (many diffs) |
| 4 | mouse | 56310 | Gps2 | G protein pathway suppressor 2 | XM_017314685.1 | 84.5% | 90.3% | (many diffs) |
| 5 | mouse | 56310 | Gps2 | G protein pathway suppressor 2 | XM_006533785.2 | 78.7% | 78.4% | (many diffs) |
| 6 | mouse | 56310 | Gps2 | G protein pathway suppressor 2 | XM_017314684.1 | 73.7% | 73.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgcactcctg gagcgcccca agctttccaa cgccatggcc agggcgctgc 121 accggcacat tatgatggag cgggagcgca agcggcagga ggaagaagag gtggataaga 181 tgatggaaca gaagatgaag gaagaacagg agagaaggaa gaaaaaggag atggaagaga 241 gaatgtcatt agaggagacc aaggaacaaa ttctgaagtt ggaggagaag cttttggctc 301 tacaggaaga gaagcaccag cttttcctgc agctcaagaa agttttacat gaggaagaaa 361 aacggaggcg aaaggaacag agtgacctga ccaccctaac atcagctgca taccagcaga 421 gcctgactgt tcacacagga actcatctcc tcagcatgca ggggagccct ggaggacaca 481 atcgcccagg caccctcatg gcagctgaca gagccaaaca aatgtttgga ccccaagtgc 541 ttacgacccg gcactacgtg ggctcagcag ctgcttttgc agggacacca gagcatggac 601 aattccaagg cagtcctggt ggtgcctatg ggactgctca gcccccacct cactatgggc 661 ccacacagcc agcttatagt cctagtcagc agctcagagc tccttcggca ttccctgcag 721 tgcagtacct atcTCAGCCA CAGCCACAGC CCTATGCTGT GCATGGCCAC TTTCAGCCCA 781 CTCAGACAGG TTTCCTCCAG CCTGGTGGTG CCCTGTCCTT GCAAAAGCAG ATGGAACATG 841 CTAACCAGCA GACTGGCTTC TCCGACTCAT CCTCTCTGCG CCCCATGCAC CCCCAGGCTC 901 TGCATCCAGC CCCTGGACTC CTTGCTTCCC CCCAGCTCCC TGTGCAGATG CAGCCAGCAG 961 GAAAGTCGGG CTTTGCAGCT ACCAGCCAAC CTGGCCCTCG GCTCCCCTTC ATCCAACACA 1021 GCCAGAACCC GCGATTCTAC CACAAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATTTCCTG 1201 CGCAACTCTC GGAACCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt