Construct: ORF TRCN0000466252
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006326.1_s317c1
- Derived from:
- ccsbBroadEn_06722
- DNA Barcode:
- AACGCGGCGTAGACCCCGGAAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERPINB6 (5269)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466252
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5269 | SERPINB6 | serpin family B member 6 | NM_001271824.1 | 99.9% | 99.7% | 268A>G |
| 2 | human | 5269 | SERPINB6 | serpin family B member 6 | NM_001271825.2 | 99.9% | 99.7% | 268A>G |
| 3 | human | 5269 | SERPINB6 | serpin family B member 6 | NM_001297699.1 | 99.9% | 99.7% | 268A>G |
| 4 | human | 5269 | SERPINB6 | serpin family B member 6 | NM_001297700.1 | 99.9% | 99.7% | 268A>G |
| 5 | human | 5269 | SERPINB6 | serpin family B member 6 | NM_004568.5 | 99.9% | 99.7% | 268A>G |
| 6 | human | 5269 | SERPINB6 | serpin family B member 6 | XM_011514674.2 | 99.9% | 99.7% | 268A>G |
| 7 | human | 5269 | SERPINB6 | serpin family B member 6 | XM_024446464.1 | 99.9% | 99.7% | 268A>G |
| 8 | human | 5269 | SERPINB6 | serpin family B member 6 | NM_001195291.2 | 98.8% | 98.6% | 1_12del;280A>G |
| 9 | human | 5269 | SERPINB6 | serpin family B member 6 | XM_017010940.1 | 98.8% | 98.6% | 1_12del;280A>G |
| 10 | human | 5269 | SERPINB6 | serpin family B member 6 | XM_024446462.1 | 98.8% | 98.6% | 1_12del;280A>G |
| 11 | human | 5269 | SERPINB6 | serpin family B member 6 | XM_024446463.1 | 98.8% | 98.6% | 1_12del;280A>G |
| 12 | human | 5269 | SERPINB6 | serpin family B member 6 | NM_001271822.2 | 96.3% | 96.1% | 1_42del;310A>G |
| 13 | human | 5269 | SERPINB6 | serpin family B member 6 | NM_001271823.1 | 95.1% | 94.9% | 1_57del;325A>G |
| 14 | human | 5269 | SERPINB6 | serpin family B member 6 | XM_011514676.2 | 87.5% | 85.9% | (many diffs) |
| 15 | human | 5269 | SERPINB6 | serpin family B member 6 | XM_017010941.1 | 87.5% | 85.9% | (many diffs) |
| 16 | human | 5269 | SERPINB6 | serpin family B member 6 | XM_024446465.1 | 87.5% | 85.9% | (many diffs) |
| 17 | human | 5269 | SERPINB6 | serpin family B member 6 | XM_011514672.1 | 82.7% | 82.5% | 1_234del;502A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgttctcgca gaagcaaatg gcacctttgc cttaaacctt ttgaaaacgc 121 tgggtaaaga caactcgaag aatgtgtttt tctcacccat gagcatgtcc tgtgccctgg 181 ccatggtcta catgggggca aagggaaaca ccgctgcaca gatggcccag atactttctt 241 tcaataaaag tggcggtggt ggagacatcc accagggctt ccagtctctt ctcaccgaag 301 tgaacaagac tggcacgcag tacttgctta gggtggccaa caggctcttt ggggaaaagt 361 cttgtgattt cctctcatct tttagagatt cctgccaaaa attctaccaa gcagagatgg 421 aggagcttga ctttatcagc gccgtagaga agtccagaaa acacataaac acctgggtag 481 ctgaaaagac agaaggtaaa attgcggagt tgctctctcc gggctcagtg gatccattga 541 caaggctggt tctggtgaat gctgtctatt tcagaggaaa ctgggatgaa cagtttgaca 601 aggagaacac cgaggagaga ctgtttaaag tcagcaagaa tgaggagaaa cctgtgcaaa 661 tgatgtttaa gcaatctact tttaagaaga cctatatagg agaaatattt acccaaatct 721 tggtgcttcc atatgttggc aaggaactga atatgatcat catgcttccg gacgagacca 781 ctgacttgag aacggtgGAG AAAGAACTCA CTTACGAGAA GTTCGTAGAA TGGACGAGGC 841 TGGACATGAT GGATGAAGAG GAGGTGGAAG TGTCCCTCCC GCGGTTTAAA CTAGAGGAAA 901 GCTACGACAT GGAGAGTGTC CTGCGCAACC TGGGCATGAC TGATGCCTTC GAGCTGGGCA 961 AGGCAGACTT CTCTGGAATG TCCCAGACAG ACCTGTCTCT GTCCAAGGTC GTGCACAAGT 1021 CTTTTGTGGA GGTCAATGAG GAAGGCACGG AGGCTGCAGC CGCCACAGCT GCCATCATGA 1081 TGATGCGGTG TGCCAGATTC GTCCCCCGCT TCTGCGCCGA CCACCCCTTC CTTTTCTTCA 1141 TCCAGCACAG CAAGACCAAC GGGATTCTCT TCTGCGGCCG CTTTTCCTCT CCGTGCCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAA ACGCGGCGTA GACCCCGGAA GCTACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att