Construct: ORF TRCN0000466258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010086.1_s317c1
- Derived from:
- ccsbBroadEn_13541
- DNA Barcode:
- TGGCTGTGACAGCTATGTGAAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLJ33534 (285150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466258
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285150 | FLJ33534 | uncharacterized LOC285150 | NR_040080.1 | 19.9% | 1_366del;525C>T;694_1635del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 396
- ORF length:
- 327
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GGGATTACTG AGTCAGAGGA AGTGGACATT GAGTGGCTCT CAACAGACAG 121 GCTGTGTTGC TCTCACAGTG CCCAGCTTCC CATGGGTGGC CAGCCGAATG CATTATGGGA 181 GAAAGCAGGT CTCGTGGATA ATATTTCTGA AGATCGGAGC AGGTTGTCAG GTCCACGTCG 241 GCCATGACTG CAGCACGCTC CGGAGGCAGC AGGGGGCACC CTGGAGCTTC GCATCCTCCT 301 TCAGGCCCGC GGCATCTCCC CTGGCTCCAC CATCCCCAGG TGTCTCAGCA GCAGCCTTGT 361 GGCTGTGCCT CTTCTGTGCC CTCCGAGTTC TAGGCTTGCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA AGTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 ATGGCTGTGA CAGCTATGTG AAGCGACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt