Construct: ORF TRCN0000466260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014588.1_s317c1
- Derived from:
- ccsbBroadEn_09179
- DNA Barcode:
- ACAACACCCACTGATGGAATCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHF6 (84295)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466260
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84295 | PHF6 | PHD finger protein 6 | NM_032335.3 | 99.7% | 99.3% | 13G>A;377T>C |
| 2 | human | 84295 | PHF6 | PHD finger protein 6 | NM_001015877.2 | 80.9% | 76.8% | (many diffs) |
| 3 | human | 84295 | PHF6 | PHD finger protein 6 | NM_032458.3 | 80.9% | 76.8% | (many diffs) |
| 4 | mouse | 70998 | Phf6 | PHD finger protein 6 | NM_027642.2 | 74.9% | 75.6% | (many diffs) |
| 5 | mouse | 70998 | Phf6 | PHD finger protein 6 | NM_001290546.1 | 54.5% | 54.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1002
- ORF length:
- 936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aagctcaatt gaacagaaaa aagggcctac aagacagcgc aaatgtggct 121 tttgtaagtc aaatagagac aaggaatgtg gacagttact aatatctgaa aaccagaagg 181 tggcagcgca ccataagtgc atgctctttt catctgcttt ggtatcatca cactctgata 241 atgaaagtct tggtggattt tctattgaag atgtccaaaa ggaaattaaa agaggcacga 301 agctgatgtg ttctttgtgc cattgtcctg gagcaacaat tggttgtgat gtgaaaacat 361 gtcacaggac ataccactac cactgtgcat tgcatgataa agctcaaata cgagagaaac 421 cttcacaagg aatttacatg gcctattgcc gaaaacacaa gaaaactgca cataactccg 481 aagcagctga tttagaagaa agttttaatg aacatgaact ggagccctca tcacctaaaa 541 gtaaaaagaa aagtcgcaaa ggaaggccaa gaaaaactaa ttttaaaggg ctgtcagaag 601 ataccaggtc cacaTCCTCC CATGGAACAG ATGAAATGGA AAGTAGTTCC TATAGAGATA 661 GGTCTCCACA CAGAAGCAGC CCTAGTGACA CCAGGCCTAA ATGTGGATTT TGCCATGTAG 721 GGGAGGAAGA AAATGAAGCA CGAGGAAAAC TGCATATATT TAATGCCAAG AAGGCAGCTG 781 CCCATTATAA GTGCATGTTG TTTTCTTCTG GCACAGTCCA GCTCACAACA ACATCAAGAG 841 CAGAATTTGG AGACTTTGAT ATTAAAACTG TACTTCAGGA GATTAAACGA GGAAAAAGAA 901 TGGTCTGTAG TTTTTATATT TGTTATGCAA CATTACACTT GATTTGCTGC TTTAAATTTA 961 GAGTACATCC CAAATTTATC CAGTCATCAG AAAATTTAAA GTACCCAACT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGAACA ACACCCACTG ATGGAATCTC CACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t