Construct: ORF TRCN0000466329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002565.1_s317c1
- Derived from:
- ccsbBroadEn_00410
- DNA Barcode:
- CAAGTTTAACCACGTTAGCTCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2J2 (1573)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1573 | CYP2J2 | cytochrome P450 family 2 su... | NM_000775.4 | 100% | 100% | |
| 2 | human | 1573 | CYP2J2 | cytochrome P450 family 2 su... | NR_134982.1 | 72.4% | 1_52del;1242_1392del;1710_2078del | |
| 3 | human | 1573 | CYP2J2 | cytochrome P450 family 2 su... | NR_134981.1 | 68.3% | 1_52del;1050_1051ins188;1371_1739del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1572
- ORF length:
- 1506
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cgcggcgatg ggctctctgg cggctgccct ctgggcagtg gtccatcctc 121 ggactctcct actgggcact gtcgcctttc tgctcgctgc tgactttctc aaaagacggc 181 gcccaaagaa ctacccgccg gggccctggc gcctgccctt ccttggcaac ttcttccttg 241 tggacttcga gcagtcgcac ctggaggttc agctgtttgt gaagaaatat gggaaccttt 301 ttagcttgga gcttggtgac atatctgcag ttcttattac tggcttgccc ttaatcaaag 361 aagcccttat ccacatggac caaaactttg ggaaccgccc cgtgacccct atgcgagaac 421 atatctttaa gaaaaatgga ttgattatgt caagtggcca ggcatggaag gagcaaagaa 481 ggttcactct gacagcacta aggaactttg gtttaggaaa gaagagctta gaggaacgca 541 ttcaggagga ggcccaacac ctcactgaag caataaaaga ggagaacgga cagccttttg 601 accctcattt caagatcaac aatgcagttt ccaatatcat ttgctccatc accttcggag 661 aacgctttga gtaccaggat agttggtttc agcagctgct gaagttacta gatgaagtca 721 catacttgga ggcttcaaag acatgccagc tctacaatgt ctttccatgg ataatgaaat 781 tcctgcctgg accccaccaa actctcttca gcaactggaa aaaactgaaa ttgtttgttt 841 ctcatatgat tgacaaacac agaaaggatt ggaatcctgc agaaacaaga gactttattg 901 atgcttacct taaagaaatg tcaaagcaca caggcaatcc tacttcaagt ttccatgaag 961 aaaacctcat ctgcagcacc ctggacctct tctttgccgg aaccgagaca acttccacaa 1021 ctctgcgatg ggctctgctt tatatggccc tctacccaga aatccaagaa aaagtacaag 1081 ctgagattga cagagtgatt ggccaggggc agcagccgag cacagccgcc cgggagtcca 1141 tgccctacac caatgctgtc atccatgagg tgcagagaat gggcaacatc aTCCCCCTGA 1201 ACGTTCCCAG GGAAGTGACA GTTGATACCA CTTTGGCTGG GTACCACCTG CCCAAGGGTA 1261 CCATGATCCT GACCAATTTG ACGGCGCTGC ACAGGGACCC CACAGAGTGG GCCACCCCTG 1321 ACACATTCAA TCCGGACCAT TTTCTGGAGA ATGGACAGTT TAAGAAAAGG GAAGCCTTTA 1381 TGCCTTTCTC AATAGGAAAG CGGGCATGCC TCGGAGAACA GTTGGCCAGG ACTGAGCTGT 1441 TTATTTTCTT CACTTCCCTT ATGCAAAAAT TTACCTTCAG GCCCCCAAAC AATGAGAAGC 1501 TGAGCCTGAA GTTTAGAATG GGTATCACCA TTTCCCCAGT CAGTCACCGC CTCTGCGCTG 1561 TTCCTCAGGT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1621 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1681 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAA GTTTAACCAC GTTAGCTCTG 1741 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t