Construct: ORF TRCN0000466340
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010721.1_s317c1
- Derived from:
- ccsbBroadEn_05393
- DNA Barcode:
- AGCCGATGTTGGCGCCACTGGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXW12 (285231)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466340
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285231 | FBXW12 | F-box and WD repeat domain ... | NM_001159927.1 | 100% | 100% | |
| 2 | human | 285231 | FBXW12 | F-box and WD repeat domain ... | NM_207102.2 | 84.9% | 84.9% | 405_614del |
| 3 | human | 285231 | FBXW12 | F-box and WD repeat domain ... | XM_017006224.1 | 84.9% | 84.9% | 405_614del |
| 4 | human | 285231 | FBXW12 | F-box and WD repeat domain ... | NM_001159929.1 | 78.4% | 78.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1251
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagatccga ttgcctgact tagctttgaa gcgaatcttc tctttcctgg 121 acctgttcgg cttgctgcag gtttcccagg tgaacaagca ttggaatagg attgcagaca 181 gtgattacct gtggaggtca ctatctctgc agagatggga ctgcagcaac ttcaccaatc 241 aacacctggg cacacacaca tggaagcaat ttttcctgca tcaaagaagg aaggagcttc 301 ggttggcatt ggcacagccg cataacttta tctacaaagt aactaagaac atcgcatttg 361 agacggagtt ggcttatctc tcaggaaata gacttacagt ggatgaacag gagaaatcaa 421 tcatttgtag tgtatctcca aagcaagagc tttgtgcctg ggatgtgcaa gaggttggcg 481 atgctgcagg tgacatctac acatttacac tgcctgggtt aagagatgtt tctaaagtta 541 ctgcatttca atatggtatt gtacttctac actgctctcc tgacaagaaa tgggtatttg 601 catgtgggac atacagtcgt accttgccac aggtattcct cacagagtcc ttactgagac 661 catcagaagg cagtgttcct ctgtctacct ttctcccaca taaattatgt gccagcgcct 721 gctggacccc aaaggtgaaa aacaggataa cactgatgtc ccaaagtagc actggaaaaa 781 agacagaatt tatcaccttt gatctaacaa ccaagaagac tggaggccaa acagtcatcc 841 aagcatatga gatcgcaagt ttccaggtgg cagctcatct gaagtgccct atctggatgg 901 gagccagtga tGGATATATG ATTGTCTTTA CCAGTGGACC ATACTTGTTA CTCTTCAGCA 961 TCACTGGCTT CCTGCTGCAA CGATTTGAGG ACCATCAGGC AGCCATCAAC AACTTCTGGG 1021 TGGATCCTTG CTATGTGCTC ACCACATCCG AGAACTCTGT GCACGTGTAC ATGTGGGAAG 1081 AAGGAGGCCG CCATCCATAC CTCAGGAGCT GCTGTCACCT GGAAAACACG TGGCATGATC 1141 ACACAACAGA CAGCTGCATC TCCAGTGTGA TGTGTGATAA TGCAAGCATA GTACTTAGGG 1201 TGAGGAAAGT AAGTGACTCC AGCATTCTGG TGATGTATTC TTTGAATACG TTGCCAACTT 1261 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1321 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1381 CTTGTGGAAA GGACGAAGCC GATGTTGGCG CCACTGGTCA ACGCGTTAAG TCgacaatca 1441 acctctggat tacaaaattt gtgaaagatt