Transcript: Human NM_207102.2

Homo sapiens F-box and WD repeat domain containing 12 (FBXW12), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
FBXW12 (285231)
Length:
1647
CDS:
187..1581

Additional Resources:

NCBI RefSeq record:
NM_207102.2
NBCI Gene record:
FBXW12 (285231)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_207102.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000431688 TAACTAAGAACATCGCATTTG pLKO_005 458 CDS 100% 10.800 15.120 N FBXW12 n/a
2 TRCN0000118462 CCGCATAACTTTATCTACAAA pLKO.1 436 CDS 100% 5.625 7.875 N FBXW12 n/a
3 TRCN0000118463 GCCATCACTATGGATCGGAAA pLKO.1 667 CDS 100% 4.050 5.670 N FBXW12 n/a
4 TRCN0000118464 CCTGACAAGAAATGGGTATTT pLKO.1 907 CDS 100% 13.200 9.240 N FBXW12 n/a
5 TRCN0000425117 TAGGATTGCAGACAGTGATTA pLKO_005 285 CDS 100% 13.200 9.240 N FBXW12 n/a
6 TRCN0000118465 CAAACAGTCATCCAAGCATAT pLKO.1 1156 CDS 100% 10.800 7.560 N FBXW12 n/a
7 TRCN0000426807 GGCTTCCTGCTGCAACGATTT pLKO_005 1294 CDS 100% 10.800 7.560 N FBXW12 n/a
8 TRCN0000121955 CTTTGATCTAACAACCAAGAA pLKO.1 1125 CDS 100% 4.950 3.465 N FBXW12 n/a
9 TRCN0000118466 GTCCCAAAGTAGCACTGGAAA pLKO.1 1086 CDS 100% 4.950 3.465 N FBXW12 n/a
10 TRCN0000412601 TAATGCAAGCATAGTACTTAG pLKO_005 1506 CDS 100% 10.800 6.480 N FBXW12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_207102.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05393 pDONR223 100% 84.9% 84.9% None 405_614del n/a
2 ccsbBroad304_05393 pLX_304 0% 84.9% 84.9% V5 405_614del n/a
3 TRCN0000466340 AGCCGATGTTGGCGCCACTGGTCA pLX_317 29% 84.9% 84.9% V5 405_614del n/a
4 ccsbBroadEn_13544 pDONR223 100% 54.4% 54.5% None (many diffs) n/a
5 ccsbBroad304_13544 pLX_304 0% 54.4% 54.5% V5 (many diffs) n/a
6 TRCN0000476240 CTTCAACCATAGCAGAAAAGTGCA pLX_317 42.8% 54.4% 54.5% V5 (many diffs) n/a
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