Construct: ORF TRCN0000466350

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011758.1_s317c1
Derived from:
ccsbBroadEn_08595
DNA Barcode:
AGCACCGTACTCTTATGAAATACT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EMC3 (55831)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466350
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55831 EMC3 ER membrane protein complex... NM_018447.3 99.8% 100% 52C>T
2 human 55831 EMC3 ER membrane protein complex... XM_005265321.3 88.3% 80.2% (many diffs)
3 mouse 66087 Emc3 ER membrane protein complex... NM_175101.3 91.3% 99.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
849
ORF length:
783
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc agggccagaa ctgttgctcg actccaacat ccgcctctgg gtggtcttac
121ccatcgttat catcactttc ttcgtaggca tgatccgcca ctacgtgtcc atcctgctgc
181agagcgacaa gaagctcacc caggaacaag tatctgacag tcaagtccta attcgaagca
241gagtcctcag ggaaaatgga aaatacattc ccaaacagtc tttcttgaca cgaaaatatt
301atttcaacaa cccagaggat ggatttttca aaaaaactaa acggaaggta gtgccacctt
361ctcctatgac tgatcctact atgttgacag acatgatgaa agggaatgta acaaatgtcc
421tccctatgat tcttattggt ggatggatca acatgacatt ctcaggcttt gtcacaacca
481aggtcccatt tccactgacc ctccgtttta agcctatgtt acagcaagga atcgagctac
541tcacattaga tgcatcctgg gtgagttctg catcctggta cttcctcaat gtatttgggc
601ttcggagcat ttactctctg attcTGGGCC AAGATAATGC CGCTGACCAA TCACGAATGA
661TGCAGGAGCA GATGACGGGA GCAGCCATGG CCATGCCCGC AGACACAAAC AAAGCTTTCA
721AGACAGAGTG GGAAGCTTTG GAGCTGACGG ATCACCAGTG GGCACTAGAT GATGTCGAAG
781AAGAGCTCAT GGCCAAAGAC CTCCACTTCG AAGGCATGTT CAAAAAGGAA TTACAGACCT
841CTATTTTTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
901CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGCCC GTAACTTGAA AGTATTTCGA
961TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCACC GTACTCTTAT GAAATACTAC
1021GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)