Construct: ORF TRCN0000466350
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011758.1_s317c1
- Derived from:
- ccsbBroadEn_08595
- DNA Barcode:
- AGCACCGTACTCTTATGAAATACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EMC3 (55831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466350
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55831 | EMC3 | ER membrane protein complex... | NM_018447.3 | 99.8% | 100% | 52C>T |
| 2 | human | 55831 | EMC3 | ER membrane protein complex... | XM_005265321.3 | 88.3% | 80.2% | (many diffs) |
| 3 | mouse | 66087 | Emc3 | ER membrane protein complex... | NM_175101.3 | 91.3% | 99.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agggccagaa ctgttgctcg actccaacat ccgcctctgg gtggtcttac 121 ccatcgttat catcactttc ttcgtaggca tgatccgcca ctacgtgtcc atcctgctgc 181 agagcgacaa gaagctcacc caggaacaag tatctgacag tcaagtccta attcgaagca 241 gagtcctcag ggaaaatgga aaatacattc ccaaacagtc tttcttgaca cgaaaatatt 301 atttcaacaa cccagaggat ggatttttca aaaaaactaa acggaaggta gtgccacctt 361 ctcctatgac tgatcctact atgttgacag acatgatgaa agggaatgta acaaatgtcc 421 tccctatgat tcttattggt ggatggatca acatgacatt ctcaggcttt gtcacaacca 481 aggtcccatt tccactgacc ctccgtttta agcctatgtt acagcaagga atcgagctac 541 tcacattaga tgcatcctgg gtgagttctg catcctggta cttcctcaat gtatttgggc 601 ttcggagcat ttactctctg attcTGGGCC AAGATAATGC CGCTGACCAA TCACGAATGA 661 TGCAGGAGCA GATGACGGGA GCAGCCATGG CCATGCCCGC AGACACAAAC AAAGCTTTCA 721 AGACAGAGTG GGAAGCTTTG GAGCTGACGG ATCACCAGTG GGCACTAGAT GATGTCGAAG 781 AAGAGCTCAT GGCCAAAGAC CTCCACTTCG AAGGCATGTT CAAAAAGGAA TTACAGACCT 841 CTATTTTTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGCCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCACC GTACTCTTAT GAAATACTAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt