Construct: ORF TRCN0000466356
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006052.1_s317c1
- Derived from:
- ccsbBroadEn_05132
- DNA Barcode:
- GTAGACCCTATCTTCCATAATCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CABP7 (164633)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466356
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 164633 | CABP7 | calcium binding protein 7 | NM_182527.3 | 100% | 100% | |
| 2 | human | 164633 | CABP7 | calcium binding protein 7 | XM_006724161.4 | 98.1% | 98.1% | 364_365insAGTGCGACATGC |
| 3 | mouse | 192650 | Cabp7 | calcium binding protein 7 | NM_138948.3 | 94.1% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gttccacccg gtgacggcgg cgttgatgta ccggggcatc tacaccgtgc 121 ccaacctgct gtcggagcag cgcccggtgg acatcccgga ggacgagctg gaggagatcc 181 gagaggcctt caaggtgttt gaccgtgacg gcaatggctt catctccaag caggagctgg 241 gcacagccat gcgctcactg ggttacatgc ccaacgaggt ggagctggag gtcaTCATCC 301 AGCGGCTGGA CATGGATGGT GATGGTCAAG TGGACTTTGA GGAGTTTGTG ACCCTTCTGG 361 GACCCAAACT CTCCACCTCA GGGATCCCAG AGAAGTTCCA TGGCACCGAC TTTGATACTG 421 TCTTCTGGAA GTGCGACATG CAGAAGCTGA CGGTGGATGA GCTGAAGCGG CTGCTCTACG 481 ACACCTTCTG CGAGCACCTG TCCATGAAGG ACATAGAGAA CATCATCATG ACGGAGGAGG 541 AGAGCCACCT GGGCACAGCC GAGGAGTGTC CCGTGGATGT GGAGACCTGC TCCAACCAGC 601 AGATCCGCCA GACTTGCGTG CGCAAGAGTC TCATCTGCGC CTTCGCCATC GCCTTCATCA 661 TCAGTGTCAT GCTCATTGCG GCCAACCAGG TGCTGCGCAG TGGCATGAAG TACCCAACTT 721 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 781 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 841 CTTGTGGAAA GGACGAGTAG ACCCTATCTT CCATAATCCG ACGCGTTAAG TCgacaatca 901 acctctggat tacaaaattt gtgaaagatt