Construct: ORF TRCN0000466362
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014622.1_s317c1
- Derived from:
- ccsbBroadEn_15592
- DNA Barcode:
- CTCCACGGCACCAAGATTTTCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC1A7 (6512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466362
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6512 | SLC1A7 | solute carrier family 1 mem... | NM_001287596.2 | 100% | 100% | |
| 2 | human | 6512 | SLC1A7 | solute carrier family 1 mem... | NM_006671.6 | 27.6% | 26.4% | (many diffs) |
| 3 | human | 6512 | SLC1A7 | solute carrier family 1 mem... | NM_001287595.2 | 24.9% | 23.9% | (many diffs) |
| 4 | human | 6512 | SLC1A7 | solute carrier family 1 mem... | NR_109858.2 | 17.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gccgcatgcc atcttggcac gggggaggga cgtgtgcagg cggaatggac 121 tcctcatcct gtctgtgctg tctgtcatcg tgggctgcct cctcggcttc ttcttgagga 181 cccggcgcct ctcaccacag gaaattagtt acttccagtt ccctggagag ctccTGATGA 241 GGATGCTGAA GATGATGATC CTGCCACTGG TGGTCTCCAG CTTGATGTCC GGACTTGCCT 301 CCCTGGATGC CAAGACCTCT AGCCGCCTGG GCGTCCTCAC CGTGGCGTAC TACCTGTGGA 361 CCACCTTCAT GGCTGTCATC GTGGGCATCT TCATGGTCTC CATCATCCAC CCAGGCAGCG 421 CGGCCCAGAA GGAGACCACG GAGCAGAGTG GGAAGCCCAT CATGAGCTCA GCCGATGCCC 481 TGTTGGACCT CATCCGGCAG AAAGAAGAAA GTTGGAGGAA CGGACCAAAG GGTCCTGGCT 541 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TTGTGGAAAG GACGACTCCA CGGCACCAAG ATTTTCTAGA CGCGTTAAGT 721 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt