Construct: ORF TRCN0000466366
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002258.1_s317c1
- Derived from:
- ccsbBroadEn_11278
- DNA Barcode:
- ATTATTCCTATCCGAAATTATCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KMO (8564)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466366
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8564 | KMO | kynurenine 3-monooxygenase | NM_003679.5 | 83.6% | 83.5% | 1097_1135del;1259A>G;1261_1458del |
| 2 | human | 8564 | KMO | kynurenine 3-monooxygenase | XM_005273337.1 | 79.2% | 79.1% | 1_120del;1340A>G;1342_1539del |
| 3 | human | 8564 | KMO | kynurenine 3-monooxygenase | XM_005273338.3 | 67% | 66.8% | (many diffs) |
| 4 | human | 8564 | KMO | kynurenine 3-monooxygenase | XM_017002649.1 | 67% | 66.8% | (many diffs) |
| 5 | human | 8564 | KMO | kynurenine 3-monooxygenase | XM_017002650.1 | 67% | 66.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1287
- ORF length:
- 1221
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctcatctgtc attcaaagga aaaaagtagc tgtcattggt ggtggcttgg 121 ttggctcatt acaagcatgc tttcttgcaa agaggaattt ccagattgat gtatatgaag 181 ctagggaaga tactcgagtg gctaccttca cacgtggaag aagcattaac ttagcccttt 241 ctcatagagg acgacaagcc ttgaaagctg ttggcctgga agatcagatt gtatcccaag 301 gtattcccat gagagcaaga atgatccact ctctttcagg aaaaaagtct gcaattccct 361 atgggacaaa gtctcagtat attctttctg taagcagaga aaatctaaac aaggatctat 421 tgactgctgc tgagaaatac cccaatgtga aaatgcactt taaccacagg ctgttgaaat 481 gtaatccaga ggaaggaatg atcacagtgc ttggatctga caaagttccc aaagatgtca 541 cttgtgacct cattgtagga tgtgatggag cctattcaac tgtcagatct cacctgatga 601 agaaacctcg ctttgattac agtcagcagt acattcctca tgggtacatg gagttgacta 661 ttccacctaa gaacggagat tatgccatgg aacctaatta tctgcatatt tggcctagaa 721 atacctttat gatgattgca cttcctaaca tgaacaaatc attcacatgt actttgttca 781 tgccctttga agagtttgaa aaacttctaa ccagtaatga tgtggtagat ttcttccaga 841 aatactttcc ggatgccatc cctctaattg gagagaaact cctagtgcaa gatttcttcc 901 tgttgcctgc ccagcccatg atatctgtaa agtgctcttc atttcacttt aaatctcact 961 gtgtactgct gggagatgca gctcatgcta tagtgccgtt tttTGGGCAA GGAATGAATG 1021 CGGGCTTTGA AGACTGCTTG GTATTTGATG AGTTAATGGA TAAATTCAGT AACGACCTTA 1081 GTTTGTGTCT TCCTGTGTTC TCAAGATTGA GAATCCCAGA TGATCACGCG ATTTCAGACC 1141 TATCCATGTA CAATTACATA GAGAAGAACA TGGAGAGATT TCTTCATGCG ATTATGCCAT 1201 CGACCTTTAT CCCTCTCTAT ACAATGGTCA CTTTTTCCAG AATAAGATAC CATGAGGCTG 1261 TGCAGCGTTG GCATTGGCAA AAAAGGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATTATTCC 1441 TATCCGAAAT TATCGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt