Construct: ORF TRCN0000466369
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006131.1_s317c1
- Derived from:
- ccsbBroadEn_11705
- DNA Barcode:
- CAAGTAGTGCTATACTTATTTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KAZN (23254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466369
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23254 | KAZN | kazrin, periplakin interact... | NM_201628.3 | 28.6% | 28.5% | 1_1659del;2116G>A |
| 2 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_011541076.2 | 25.6% | 23.4% | (many diffs) |
| 3 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_011541077.2 | 25.6% | 23.4% | (many diffs) |
| 4 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_005245795.5 | 25.3% | 25.3% | 1_1953del;2410G>A |
| 5 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_011541075.2 | 23% | 21.1% | (many diffs) |
| 6 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_011541074.3 | 20.6% | 18.8% | (many diffs) |
| 7 | mouse | 71529 | Kazn | kazrin, periplakin interact... | NM_144531.3 | 25.1% | 25.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 735
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgaattcc ctgatgaagc gagacctgga gaagcacctg aacgtgtcca 121 agaagttcca ccaggtcagc atcctgctgg ggatcgagct gctgtaccaa gtgaacttca 181 gcagggaggc cctccaggag cgccgggccc gctgcgagac gcagaacatt gaccccgtgg 241 tgtggaccaa ccagcgggtg ctcaagtggg ttcgagacat cgacctgaag gagtacgcag 301 acaacctgac caacagcggc gtccatggtg ctgtgctggt gctggagccc acattcaatg 361 ccgaggccat ggccactgcc ctgggcatcc ccagtgggaa gcacatccTC CGGAGACACC 421 TGGCAGAGGA GATGAGCGCC GTCTTCCACC CAGCCAACTC CACAGGCATC CGGGAGGCTG 481 AGCGTTTTGG AACGCCCCCT GGCAGGGCCT CCAGCGTCAC GCGGACAGGA AAGGAGGAGA 541 ACAGCAGCGG TCTCAAGTAC AAGGCTGGCC GGCTGCCCCT GGGGAAGATA GGAAGGGGCT 601 TCAGCAGCAA AGATCCCGAT TTCCATGATG ACTATGGCTC TCTTCAAAAC GAAGATTGCG 661 GAGACGATGA CCCCCAGAGC AGGCTGGAAC AGTGCCGTCT GGAAGGCTAC AACAGCCTGG 721 AGGTCACCAA CGTGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CAAGTAGTGC TATACTTATT 901 TTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt