Construct: ORF TRCN0000466385
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003507.1_s317c1
- Derived from:
- ccsbBroadEn_06813
- DNA Barcode:
- CTTCCCTGGATGTTGGCCAAGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QSOX1 (5768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466385
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5768 | QSOX1 | quiescin sulfhydryl oxidase 1 | NM_001004128.2 | 99.7% | 99.8% | (many diffs) |
| 2 | human | 5768 | QSOX1 | quiescin sulfhydryl oxidase 1 | NM_002826.5 | 80.4% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1878
- ORF length:
- 1812
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gaggtgcaac agcggctccg ggccgccgcc gtcgctgctg ctgctgctgc 121 tgtggctgct cgcggttccc ggcgctaacg cggccccgcg gtcggcgctc tattcgcctt 181 ccgacccgct gacgctgctg caggcggaca cggtgcgcgg cgcggtgctg ggctcccgca 241 gcgcctgggc cgtggagttc ttcgcctcct ggtgcggcca ctgcatcgcc ttcgccccga 301 cgtggaaggc gctggccgaa gacgtcaaag cctggaggcc ggccctgtat ctcgccgccc 361 tggactgtgc tgaggagacc aacagtgcag tctgcagaga cttcaacatc cctggcttcc 421 cgactgtgag gttcttcaag gcctttacca agaacggctc aggagcagta tttccagtgg 481 ctggtgctga cgtgcagacg ctgcgggaga ggctcattga cgccctggag tcccatcatg 541 acacgtggcc cccagcctgt cccccactgg agcctgccaa gctggaggag attgatggat 601 tctttgcgag aaataacgaa gagtacctgg ctctgatctt tgaaaaggga ggctcctacc 661 tggctagaga ggtggctctg gacctgtccc agcacaaagg cgtggcggtg cgcagggtgc 721 tgaacacaga ggccaatgtg gtgagaaagt ttggtgtcac cgacttcccc tcttgctacc 781 tgctgttccg gaatggctct gtctcccgag tccccgtgct catggaatcc aggtccttct 841 ataccgctta cctgcagaga ctctctgggc tcaccaggga ggctgcccag accacagttg 901 caccaaccac tgctaacaag atagctccca ctgtttggaa attggcagat cgctccaaga 961 tctacatggc tgacctggaa tctgcactgc actacatcct gcggatagaa gtgggcaggt 1021 tcccggtcct ggaagggcag cgcctggtgg ccctgaaaaa gtttgtggca gtgctggcca 1081 agtatttccc tggccggccc ttagtccaga acttcctgca ctccgtgaat gaatggctca 1141 agaggcagaa gagaaataaa attccctaca gtttctttaa aactgccctg gacgacagga 1201 aagagggtgc cgttcttgcc aagaaggtga actggattgg ctgccagggg agtgagccgc 1261 atttccgggg ctttccctgc tccctgtggg ttcttttcca cttcttgact gtgcaggcag 1321 ctcggcaaaa tgtagaccac tcacaggaag cagccaaggc caaggaggtc ctcccagcca 1381 tccgaggcta cgtgcactac ttcttcggct gccgagactg cgctagccac ttcgagcaga 1441 tggctgctgc ctccatgcac cgggtgggga gtcccaacgc cgctgTCCTC TGGCTCTGGT 1501 CTAGCCACAA CAGGGTCAAT GCTCGCCTTG CAGGTGCCCC CAGCGAGGAC CCCCAGTTCC 1561 CCAAGGTGCA GTGGCCACCC CGTGAACTTT GTTCTGCCTG CCACAATGAA CGCCTGGATG 1621 TGCCCGTGTG GGACGTGGAA GCCACCCTCA ACTTCCTCAA GGCCCACTTC TCCCCAAGCA 1681 ACATCATCCT GGACTTCCCT GCAGCTGGGT CAGCTGCCCG GAGGGATGTG CAGAATGTGG 1741 CAGCCGCCCC AGAGCTGGCG ATGGGAGCCC TGGAGCTGGA AAGCCGGAAT TCAACTCTGG 1801 ACCCTGGGAA GCCTGAGATG ATGAAGTCCC CCACAAACAC CACCCCACAT GTGCCGGCTG 1861 AGGGACCTGA GCTTATTTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1921 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1981 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTTCCCT GGATGTTGGC 2041 CAAGCCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt