Construct: ORF TRCN0000466388
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014209.1_s317c1
- Derived from:
- ccsbBroadEn_12549
- DNA Barcode:
- CTCATTATCTAAGCCTCTACTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MMP28 (79148)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466388
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79148 | MMP28 | matrix metallopeptidase 28 | NM_032950.4 | 100% | 100% | |
| 2 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525231.1 | 99.2% | 98.9% | 1168_1169insTGCAAT;1170_1171insGTT |
| 3 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_017025062.1 | 96.1% | 86.5% | 998_999insAGAC;1134_1135ins41 |
| 4 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_017025063.1 | 96.1% | 86.5% | 998_999insAGAC;1134_1135ins41 |
| 5 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_017025064.1 | 96.1% | 86.5% | 998_999insAGAC;1134_1135ins41 |
| 6 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525230.1 | 93.4% | 93% | (many diffs) |
| 7 | human | 79148 | MMP28 | matrix metallopeptidase 28 | NM_024302.5 | 75.3% | 74.8% | (many diffs) |
| 8 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525232.2 | 68.9% | 65.9% | (many diffs) |
| 9 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525225.1 | 60.9% | 60.3% | (many diffs) |
| 10 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525226.2 | 59% | 58.4% | (many diffs) |
| 11 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525227.1 | 59% | 58.4% | (many diffs) |
| 12 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525228.1 | 59% | 58.4% | (many diffs) |
| 13 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525229.2 | 59% | 58.4% | (many diffs) |
| 14 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_024450943.1 | 59% | 58.4% | (many diffs) |
| 15 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_017025061.1 | 52.6% | 49.7% | (many diffs) |
| 16 | human | 79148 | MMP28 | matrix metallopeptidase 28 | NR_111988.2 | 49.8% | (many diffs) | |
| 17 | human | 79148 | MMP28 | matrix metallopeptidase 28 | NM_001032278.3 | 32.6% | 32.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1245
- ORF length:
- 1179
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cgcgcgcgtc ggcctcctgc tgcgcgccct gcagctgcta ctgtggggcc 121 acctggacgc ccagcccgcg gagcgcggag gccaggagct gcgcaaggag gcggaggcat 181 tcctagagaa gtacggatac ctcaatgaac aggtccccaa agctcccacc tccactcgat 241 tcagcgatgc catcagagcg tttcagtggg tgtcccagct acctgtcagc ggcgtgttgg 301 accgcgccac cctgcgccag atgactcgtc cccgctgcgg ggttacagat accaacagtt 361 atgcggcctg ggctgagagg atcagtgact tgtttgctag acaccggacc aaaatgaggc 421 gtaagaaacg ctttgcaaag caaggtaaca aatggtacaa gcagcacctc tcctaccgcc 481 tggtgaactg gcctgagcat ctgccggagc cggcagttcg gggcgccgtg cgcgccgcct 541 tccagttgtg gagcaacgtc tcagcgctgg agttctggga ggccccagcc acaggccccg 601 ctgacatccg gctcaccttc ttccaagggg accacaacga tgggctgggc aatgcctttg 661 atggcccagg gggcgccctg gcgcacgcct tcctgccccg ccgcggcgaa gcgcacttcg 721 accaagatga gcgctggtcc ctgagccgcc gccgcgggcg caacctgttc gtggtgctgg 781 cgcacgagat cggtcacacg cttggcctca cccactcgcc cgcgccgcgc gcgctcatgg 841 cgcccTACTA CAAGAGGCTG GGCCGCGACG CGCTGCTCAG CTGGGACGAC GTGCTGGCCG 901 TGCAGAGCCT GTATGGGAAG CCCCTAGGGG GCTCAGTGGC CGTCCAGCTC CCAGGAAAGC 961 TGTTCACTGA CTTTGAGACC TGGGACTCCT ACAGCCCCCA AGGAAGGCGC CCTGAAACGC 1021 AGGGCCCTAA ATACTGCCAC TCTTCCTTCG ATGCCATCAC TGTAGACAGG CAACAGCAAC 1081 TGTACATTTT TAAAGGGAGC CATTTCTGGG AGGTGGCAGC TGATGGCAAC GTCTCAGAGC 1141 CCCGTCCACT GCAGGAAAGA TGGGTCGGGC TGCCCCCCAA CATTGAGGCT GCGGCAGTGT 1201 CATTGAATGA TGGAGATTTC TACTTCTTCA AAGTGCAATC CGTTTGCCCA ACTTTCTTGT 1261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1381 GAAAGGACGA CTCATTATCT AAGCCTCTAC TTTCACGCGT TAAGTCgaca atcaacctct 1441 ggattacaaa atttgtgaaa gatt