Construct: ORF TRCN0000466390
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005813.1_s317c1
- Derived from:
- ccsbBroadEn_06579
- DNA Barcode:
- AAGGCTTGCCAACCGTAACATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFGE8 (4240)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466390
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4240 | MFGE8 | milk fat globule-EGF factor... | NM_001114614.3 | 99.8% | 99.7% | 226C>A;267G>A |
| 2 | human | 4240 | MFGE8 | milk fat globule-EGF factor... | NM_005928.4 | 86.3% | 86.3% | 226C>A;267G>A;869_1024del |
| 3 | human | 4240 | MFGE8 | milk fat globule-EGF factor... | XM_024449930.1 | 80.5% | 77.1% | (many diffs) |
| 4 | human | 4240 | MFGE8 | milk fat globule-EGF factor... | NM_001310320.2 | 80.2% | 78.3% | (many diffs) |
| 5 | human | 4240 | MFGE8 | milk fat globule-EGF factor... | XR_931838.3 | 79.1% | (many diffs) | |
| 6 | human | 4240 | MFGE8 | milk fat globule-EGF factor... | NM_001310319.2 | 75% | 74.6% | (many diffs) |
| 7 | human | 4240 | MFGE8 | milk fat globule-EGF factor... | XM_017022206.2 | 70.3% | 68.6% | (many diffs) |
| 8 | human | 4240 | MFGE8 | milk fat globule-EGF factor... | NM_001310321.2 | 57.6% | 57.6% | 0_1ins336;533_688del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1071
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gcgcccccgc ctgctggccg cgctgtgcgg cgcgctgctc tgcgccccca 121 gcctcctcgt cgccctggat atctgttcca aaaacccctg ccacaacggt ggtttatgcg 181 aggagatttc ccaagaagtg cgaggagatg tcttcccctc gtacacctgc acgtgcctta 241 agggctacgc gggcaaccac tgtgagacga aatgtgtcga gccactgggc atggagaatg 301 ggaacattgc caactcacag atcgccgcct catctgtgcg tgtgaccttc ttgggtttgc 361 agcattgggt cccggagctg gcccgcctga accgcgcagg catggtcaat gcctggacac 421 ccagcagcaa tgacgataac ccctggatcc aggtgaacct gctgcggagg atgtgggtaa 481 caggtgtggt gacgcagggt gccagccgct tggccagtca tgagtacctg aaggccttca 541 aggtggccta cagccttaat ggacacgaat tcgatttcat ccatgatgtt aataaaaaac 601 acaaggagtt tgtgggtaac tggaacaaaa acgcggtgca tgtcaacctg tttgagaccc 661 ctgtggaggc tcagtacgtg agattgtacc ccacgagctg ccacacggcc TGCACTCTGC 721 GCTTTGAGCT ACTGGGCTGT GAGCTGAACG GATGCGCCAA TCCCCTGGGC CTGAAGAATA 781 ACAGCATCCC TGACAAGCAG ATCACGGCCT CCAGCAGCTA CAAGACCTGG GGCTTGCATC 841 TCTTCAGCTG GAACCCCTCC TATGCACGGC TGGACAAGCA GGGCAACTTC AACGCCTGGG 901 TTGCGGGGAG CTACGGTAAC GATCAGTGGC TGCAGATCTT CCCTGGCAAC TGGGACAACC 961 ACTCCCACAA GAAGAACTTG TTTGAGACGC CCATCCTGGC TCGCTATGTG CGCATCCTGC 1021 CTGTAGCCTG GCACAACCGC ATCGCCCTGC GCCTGGAGCT GCTGGGCTGT TACCCAACTT 1081 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1141 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1201 CTTGTGGAAA GGACGAAAGG CTTGCCAACC GTAACATTCA ACGCGTTAAG TCgacaatca 1261 acctctggat tacaaaattt gtgaaagatt