Construct: ORF TRCN0000466407
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006858.1_s317c1
- Derived from:
- ccsbBroadEn_06233
- DNA Barcode:
- CACCGCCTGACGTTCTTTGTTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FYB1 (2533)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466407
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2533 | FYB1 | FYN binding protein 1 | NM_001349333.2 | 99.9% | 99.8% | 2152G>T |
| 2 | human | 2533 | FYB1 | FYN binding protein 1 | NM_001465.6 | 99.9% | 99.8% | 2152G>T |
| 3 | human | 2533 | FYB1 | FYN binding protein 1 | XM_006714464.3 | 99.9% | 99.8% | 2152G>T |
| 4 | human | 2533 | FYB1 | FYN binding protein 1 | XM_006714466.2 | 99.9% | 99.8% | 2152G>T |
| 5 | human | 2533 | FYB1 | FYN binding protein 1 | XM_011514009.1 | 99.9% | 99.8% | 2152G>T |
| 6 | human | 2533 | FYB1 | FYN binding protein 1 | XM_011514010.1 | 99.9% | 99.8% | 2152G>T |
| 7 | human | 2533 | FYB1 | FYN binding protein 1 | NM_001243093.2 | 98.7% | 98.6% | 1_30del;2182G>T |
| 8 | human | 2533 | FYB1 | FYN binding protein 1 | XM_011514008.3 | 98.7% | 98.6% | 1_30del;2182G>T |
| 9 | human | 2533 | FYB1 | FYN binding protein 1 | NM_199335.5 | 94.4% | 94.3% | 1907_1908ins138;2014G>T |
| 10 | human | 2533 | FYB1 | FYN binding protein 1 | NM_018594.2 | 93.2% | 93.2% | 1_30del;1937_1938ins138;2044G>T |
| 11 | human | 2533 | FYB1 | FYN binding protein 1 | XM_011514011.2 | 93.2% | 93.2% | 1_30del;1937_1938ins138;2044G>T |
| 12 | human | 2533 | FYB1 | FYN binding protein 1 | XM_011514012.3 | 81.2% | 81.2% | 1_30del;2076_2076delGins442 |
| 13 | human | 2533 | FYB1 | FYN binding protein 1 | XM_011514013.3 | 58.9% | 57.6% | (many diffs) |
| 14 | mouse | 23880 | Fyb | FYN binding protein | XM_006519986.1 | 39.4% | 39.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2556
- ORF length:
- 2487
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgaaatat aacacggggg gcaacccgac agaggatgtc tcagtcaata 121 gccgaccctt cagagtcaca gggccaaact catcttcagg aatacaagca agaaagaact 181 tattcaacaa ccaaggaaat gccagccctc ctgcaggacc cagcaatgta cctaagtttg 241 ggtccccaaa gccacctgtg gcagtcaaac cttcttctga ggaaaagcct gacaaggaac 301 ccaagccccc gtttctaaag cccactggag caggccaaag attcggaaca ccagccagct 361 tgaccaccag agaccccgag gcgaaagtgg gatttctgaa acctgtaggc cccaagccca 421 tcaacttgcc caaagaagat tccaaaccta catttccctg gcctcctgga aacaagccat 481 ctcttcacag tgtaaaccaa gaccatgact taaagccact aggcccgaaa tctgggccta 541 ctcctccaac ctcagaaaat gaacagaagc aagcgtttcc caaattgact ggggttaaag 601 ggaaatttat gtcagcatca caagatcttg aacccaagcc cctcttcccc aaacccgcct 661 ttggccagaa gccgccccta agtaccgaga actcccatga agacgaaagc cccatgaaga 721 atgtgtcttc atcaaaaggg tccccagctc ccctgggagt caggtccaaa agcggccctt 781 taaaaccagc aagggaagac tcagaaaata aagaccatgc aggggagatt tcaagtttgc 841 cctttcctgg agtggttttg aaacctgctg cgagcagggg aggcccaggt ctctccaaaa 901 atggtgaaga aaaaaaggaa gataggaaga tagatgctgc taagaacacc ttccagagca 961 aaataaatca ggaagagttg gcctcaggga ctcctcctgc caggttccct aaggcccctt 1021 ctaagctgac agtggggggg ccatggggcc aaagtcagga aaaggaaaag ggagacaaga 1081 attcagccac cccgaaacag aagccattgc ctcccttgtt taccttgggt ccacctccac 1141 caaaacccaa cagaccacca aatgttgacc tgacgaaatt ccacaaaacc tcttctggaa 1201 acagtactag caaaggccag acgtcttact caacaacttc cctgccacca cctccaccat 1261 cccatccggc cagccaacca ccattgccag catctcaccc atcacaacca ccagtcccaa 1321 gcctacctcc cagaaacatt aaacctccgt ttgacctaaa aagccctgtc aatgaagaca 1381 atcaagatgg tgtcacgcac tctgatggtg ctggaaatct agatgaggaa caagacagtg 1441 aaggagaaac atatgaagac atagaagcat ccaaagaaag agagaagaaa agggaaaagg 1501 aagaaaagaa gaggttagag ctggagaaaa aggaacagaa agagaaagaa aagaaagaac 1561 aagaaataaa gaagaaattt aaactaacag gccctattca agtcatccat cttgcaaaag 1621 cttgttgtga tgtcaaagga ggaaagaatg aactgagctt caagcaagga gagcaaattg 1681 aaatcatccg catcacagac aacccagaag gaaaatggtt gggcagaaca gcaaggggtt 1741 catatggcta tattaaaaca actgctgtag agattgacta tgattctttg aaactgaaaa 1801 aagactctct tggtgcccct tcaagaccta ttgaagatga ccaagaagta tatgatgatg 1861 ttgcagagca ggatgatatt agcagccaca gtcagagtgg aagtggaggg atattccctc 1921 caccaccaga tgatgacatt tatgatggga ttgaagagga agatgctgat gatggctcca 1981 cactacaggt tcaagagaag agtaatacgt ggtcctgggg gattttgaag atgttaaagg 2041 gaaaagatga cagaaagaaa agtatacgag agaaacctaa agtctctgac tcagacaata 2101 atgaaggttc atctttccct gctcctccta aacaattgga catgggagat gaagtttacg 2161 atgatgtgga taccTCTGAT TTCCCTGTTT CATCAGCAGA GATGAGTCAA GGAACTAATT 2221 TTGGAAAAGC TAAGACAGAA GAAAAGGACC TTAAGAAGCT AAAAAAGCAG GAAAAAGAAG 2281 AAAAAGACTT CAGGAAAAAA TTTAAATATG ATGGTGAAAT TAGAGTCCTA TATTCAACTA 2341 AAGTTACAAC TTCCATAACT TCTAAAAAGT GGGGAACCAG AGATCTACAG GTAAAACCTG 2401 GTGAATCTCT AGAAGTTATA CAAACCACAG ATGACACAAA AGTTCTCTGC AGAAATGAAG 2461 AAGGGAAATA TGGTTATGTC CTTCGGAGTT ACCTAGCGGA CAATGATGGA GAGATCTATG 2521 ATGATATTGC TGATGGCTGC ATCTATGACA ATGACTTGCC AACTTTCTTG TACAAAGTGG 2581 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2641 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2701 ACACCGCCTG ACGTTCTTTG TTAACACGCG TTAAGTCgac aatcaacctc tggattacaa 2761 aatttgtgaa agatt