Construct: ORF TRCN0000466420
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017925.1_s317c1
- Derived from:
- ccsbBroadEn_12819
- DNA Barcode:
- GCAATCTAATCTTAACTCCGACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF333 (84449)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466420
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84449 | ZNF333 | zinc finger protein 333 | NM_001300912.2 | 99.8% | 99.6% | 343T>C |
| 2 | human | 84449 | ZNF333 | zinc finger protein 333 | NM_001352244.2 | 80.9% | 80.3% | (many diffs) |
| 3 | human | 84449 | ZNF333 | zinc finger protein 333 | NM_032433.4 | 45.4% | 44.9% | (many diffs) |
| 4 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_011528362.2 | 45.4% | 44.9% | (many diffs) |
| 5 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_017027367.1 | 45.4% | 44.9% | (many diffs) |
| 6 | human | 84449 | ZNF333 | zinc finger protein 333 | NM_001352239.2 | 29% | 28.5% | (many diffs) |
| 7 | human | 84449 | ZNF333 | zinc finger protein 333 | NM_001352240.2 | 29% | 28.5% | (many diffs) |
| 8 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_011528363.2 | 29% | 28.5% | (many diffs) |
| 9 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_011528365.2 | 29% | 28.5% | (many diffs) |
| 10 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_011528367.2 | 29% | 28.5% | (many diffs) |
| 11 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_011528368.2 | 29% | 28.5% | (many diffs) |
| 12 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_011528369.2 | 29% | 28.5% | (many diffs) |
| 13 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_017027369.1 | 29% | 28.5% | (many diffs) |
| 14 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_017027370.1 | 29% | 28.5% | (many diffs) |
| 15 | human | 84449 | ZNF333 | zinc finger protein 333 | NM_001352241.2 | 23.5% | 23.1% | (many diffs) |
| 16 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_024451740.1 | 23.5% | 23.1% | (many diffs) |
| 17 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_024451741.1 | 23.5% | 23.1% | (many diffs) |
| 18 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_011528371.2 | 17% | 16.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 975
- ORF length:
- 909
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga atccgtcacc tttgaggatg tggccgtgga gttcatccag gagtgggcat 121 tgctggacag cgcacggagg agcctgtgca aatacaggat gcttgaccag tgcaggaccc 181 tggcctccag gggaactcca ccatgcaaac ccagttgtgt ctcccagctg gggcaaagag 241 cagagccaaa ggcaacagaa cgagggattc tccgtgccac aggtgttgcc tgggaatctc 301 aacttaaacc cgaagagttg ccttctatgc aggatctttt ggaagaagca tcctccaggg 361 acatgcaaat ggggccgggg ctgttcctga ggatgcagct ggtgcccccc atagaagaga 421 gggagacacc attgactcga gaggaccggc cagctctcca ggagccgcct tggtctctgg 481 gatgcacggg actgaaggcc gctatgcaga ttcagagggt ggtgatacca gtgcctactc 541 tgggccaccg caacccatgg gtggccaggg attctgctgt gcctgcacgt gaccctgcct 601 ggcttcagga ggacaaagtg gaggaagaag ctatggcTCC TGGGCTGCCA ACCGCCTGTT 661 CACAGGAACC AGTCACCTTT GCAGATGTGG CTGTGGTGTT CACCCCAGAA GAATGGGTGT 721 TTCTGGACTC TACTCAGAGG AGCCTGTATA GAGATGTGAT GCTGGAGAAC TACAGGAACC 781 TGGCCTCTGT GGCTGATCAA CTGTGCAAAC CCAATGCGTT GTCTTATTTG GAAGAAAGAG 841 GAGAGCAGTG GACCACTGAC AGGGGCGTCC TCTCAGACAC CTGTGCAGAA CCTCAGTGTC 901 AACCCCAAGA GGCAATTCCT AGCCAAGATA CTTTTACAGA GATCCTGTCC ATTGATGTGA 961 AAGGGCCACC CAACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1021 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1081 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCAATCTAAT CTTAACTCCG 1141 ACAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt