Construct: ORF TRCN0000466424
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006108.1_s317c1
- Derived from:
- ccsbBroadEn_03376
- DNA Barcode:
- CCACTCATTTAACGTTGCCGGTAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MSRB1 (51734)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466424
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51734 | MSRB1 | methionine sulfoxide reduct... | NM_016332.3 | 100% | 100% | |
| 2 | mouse | 27361 | Msrb1 | methionine sulfoxide reduct... | NM_001346668.1 | 86.7% | 91.4% | (many diffs) |
| 3 | mouse | 27361 | Msrb1 | methionine sulfoxide reduct... | NM_013759.3 | 86.7% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 348
- ORF length:
- 282
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC GTTCTGCAGC TTCTTCGGGG GCGAGGTTTT CCAGAATCAC TTTGAACCTG 121 GCGTTTACGT GTGTGCCAAG TGTGGCTATG AGCTGTTCTC CAGCCGCTCG AAGTATGCAC 181 ACTCGTCTCC ATGGCCGGCG TTCACCGAGA CCATTCACGC CGACAGCGTG GCCAAGCGTC 241 CGGAGCACAA TAGATCTGAA GCCTTGAAGG TGTCCTGTGG CAAGTGTGGC AATGGGTTGG 301 GCCACGAGTT CCTGAACGAC GGCCCCAAGC CGGGGCAGTC CCGATTCTGA ATATTCAGCA 361 GCTCGCTGAA GTTTGTCCCT AAAGGCAAAG AAACTTCTGC CTCCCAGGGT CACTACCCAA 421 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 481 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 541 TATCTTGTGG AAAGGACGAC CACTCATTTA ACGTTGCCGG TACACGCGTT AAGTCgacaa 601 tcaacctctg gattacaaaa tttgtgaaag att